摘要
目的:检测B-Raf和Raf1蛋白在犬乳腺肿瘤组织中的差异表达,明确其在犬乳腺肿瘤发生发展中的作用。方法:采用病理组织学技术对临床疑似肿瘤进行良恶性质鉴定,免疫组织化学技术检测犬乳腺肿瘤中Raf1和B-Raf蛋白的差异表达。结果:Raf1蛋白的阳性表达率为94.74%(18/19),B-Raf蛋白的阳性表达率为47.37%(9/19),两种蛋白在犬乳腺肿瘤中的表达呈显著性差异(P<0.01)。不同性质的犬乳腺肿瘤中Raf1表达未见显著性差异,恶性肿瘤中导管内癌和浸润性乳腺癌Raf1蛋白的表达呈边缘显著性差异(P=0.072)。结论:Raf1蛋白和B-Raf蛋白对犬乳腺肿瘤的发生发展起促进作用,Raf1蛋白发挥着更为广泛和重要的促进肿瘤恶性变的作用。Raf1可作为兽医临床潜在的肿瘤检测指标。
Objective:To detect the expression of Raf,including B-Raf and Raf1,in different canine mammary tumor tissues,and to explore their roles in canine mammary tumors.This study will provide some basically theoretical references for clinical diagnosis and treatment in animal tumor cases.Methods:In this study,the characteristics of tumor cases were identified by histopathology,and their different expressions of Raf1 and B-Raf in canine mammary tumor were detected by immunohistochemical staining.Results:The positive expression rate of Raf1 was 94.74%(18/19),the positive expression rate of B-Raf protein was47.37%(9/19).The expression of Raf1 in tumors were higher than B-Raf significantly(P<0.01).The expression of Raf1 in different canine mammary tumors showed no significant difference,but the expression of Raf1 in intraductal carcinoma and invasive breast cancer was marginal significant difference(P=0.072).Conclusion:Over expression of Raf1 and B-Raf may promote the development of canine mammary tumors.Raf1 plays more extensive role in malignant change of tumor,and it might be a sensitive marker applying in veterinary clinic.
出处
《北京农学院学报》
2015年第3期52-55,共4页
Journal of Beijing University of Agriculture
基金
北京市教委科研计划项目资助(PXM2013_014207_000036)
