摘要
目的实时观测SAHA和TRAIL联合使用后对乳腺癌雌激素受体阳性细胞MCF-7生长的影响。方法实时无标记动态细胞分析系统(real time cell analysis,RTCA)动态监测各种处理因素对乳腺癌MCF-7细胞生长状况的影响并通过Biostation^(TM)活细胞工作站实时收集各种处理因素对乳腺癌MCF-7细胞增殖干预的形态学证据。结果xCELLigence RTCA实时无标记动态细胞分析系统显示,随着TRAIL的加入,SAHA对MCF-7细胞的抑制作用明显增强。SAHA和TRAIL的联合用药可能提高了SAHA对MCF-7细胞的敏感性。实时活细胞工作站成像实验进一步证明SAHA和TRAIL联合用药对MCF-7乳腺癌细胞的抑制作用强于SAHA或TRAIL单独用药。结论SAHA和TRAIL的联合使用对MCF-7细胞的生长具有协同抑制作用。
Aim To investigate the effects of combined treatment of SAHA and TRAIL on human breast cancer ER positive cell line MCF-7.Methods MCF-7 cells were treated with SAHA and/or TRAIL.The inhibitory rates were detected by real-time cell proliferation assays.Morphology changes of MCF-7 cells were observed through time4 apse live cell imaging acquisition.Results Real-time cell proliferation assays showed that the anti-tumor efficacy of SAHA was significantly enhanced in combination with TRAIL.The results of time4 apse live cell imaging acquisition demonstrated that,with treatment of SAHA and TRAIL,the growth inhibition of MCF-7 cells was more obvious than that of in TRAIL or SAHA treatment alone.Conclusion The combination treatment of SAHA and TRAIL has a synergistic effect of growth inhibition on breast cancer MCF-7 cells.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2016年第2期223-228,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81172509)
沈阳医学院科技发展基金(No 20135061)
