摘要
目的 :研究克拉霉素与环丙沙星联合应用对铜绿假单胞菌生物被膜的影响。方法 :采用平板法培养细菌生物被膜 ,微量稀释法测定抗菌药物的最低抑菌浓度 ,银染法快速鉴定细菌生物被膜 ,噻唑蓝 (MTT)比色法测定生物被膜中的活菌数。结果 :1/16、1/4MIC克拉霉素可显著增加1/4、1/2MIC环丙沙星对铜绿假单胞菌生物被膜的抑菌活性。结论 :克拉霉素与环丙沙星合用对铜绿假单胞菌生物被膜具有明显的协同抑制作用。
Objective:To observe the synergetic effects of clarithromycin a nd ciprofloxacin on the bacterial biofilm (BBF) of Pseudomonas aeruginosa(PA).Me thods:MIC was determined by microdilution method.BBF was established by plate c ulture method,and it was defined by being stained with AgNO3.Viable bacteria wer e counted by using MTT method.Results:1/16 MIC and 1/4 MIC of clarithromycin c ould significantly enhance the bactericidal action of 1/4 MIC and 1/2 MIC ciprof loxacin within biofilm of PA.Conclusion:There are synergetic effects on the BB F treated with clarithromycin plus ciprofloxacin.
出处
《现代医药卫生》
2005年第3期254-255,共2页
Journal of Modern Medicine & Health
基金
浙江省医药卫生科学研究基金项目 (2003B001)
