摘要
为确定miRNA1917在植物微重力反应中的作用,对番茄培养环境中乙烯含量、与乙烯相关的表型变化以及miRNA1917及其靶基因Le CTR4相对表达量进行了测定。结果表明：模拟微重力处理条件下,番茄出现下胚轴向地性弯曲和形成螺旋等乙烯三重反应表型,丙二醛和超氧阴离子含量受模拟微重力处理影响明显,植物培养环境中乙烯含量在培养前期（10~20d）差异显著,20d以后差异不显著,miRNA1917相对表达量随处理时间持续增加,其靶基因相对表达量相应降低。由此可见,番茄下胚轴弯曲和形成螺旋等表型与模拟微重力处理初期乙烯含量有关,但在处理后期（20d以后）,MDA和O2-积累等则与miRNA1917的相对表达量变化有关,miRNA1917相对表达量增加,导致其负调控乙烯反应的靶基因相对表达量减少,从而使植物对乙烯的反应灵敏度增加,即miRNA1917通过降低其靶基因表达量而增加了植物对乙烯的反应灵敏性,从而在微重力反应过程中发挥一定作用。该研究为确立miRNA介导的植物微重力反应过程奠定了基础。
To confirm the role of miR1917 in plant response to microgravity, the ethylene level in the culture environment,the phenotypes responded to ethylene of tomatoes,as well as the expression quantity of miRNA1917 and its target gene Le CTR4 was detected. In the experiment,ethylene related phenotypes including hypocotyl bending geotropically and developing spirals were observed. The quantity of malonaldehyde（ MDA） and superoxideanion in the environment increases along the time. These reactions are clearly affected by the microgravity treatment. The difference of ethylene level in the culture environments is significant only in the initial period（ day 10 to day 20）. After 20 days,there is almost no difference. The relative expression quantity of miRNA1917 increases along the time,while the relative expression quantity of its target gene keeps decreasing. Therefore,the phenotypes of hypocotyl bending geotropically and developing spirals are related to the ethylene level in the initial microgravity treatment period. In the later period（ after20 days）,the accumulation of MDA and superoxide anion are related to the expression quantity of miRNA1917. This is because the increase of the expression quantity of miRNA1917 leads to the decrease of its target gene,which negatively controls the phenotype response to ethylene. Hence,the plant is more sensitive to the ethylene level in the environment.miRNA1917 play a role in plant microgravity response through reducing the amount of its target gene to increasing the sensitivity to ethylene,. The study laid a foundation for the establishment of plant microgravity response process mediated by miRNA.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第12期2294-2299,共6页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金面上项目(31471707)
河北省自然科学基金面上项目(D2015207013)
