摘要
目的熟悉并掌握孚尔根反应的原理及实验操作方法,观察DNA细胞的形态和结构。方法取蟾蜍血细胞涂片,60℃1NHCL水解后,用Schiff试剂染细胞核,亮绿复染细胞质。结果显微镜下观察到细胞核被染成紫红色,细胞质为绿色。结论 DNA细胞的核质红绿染色鲜明且清晰美观,让学生对生物学研究有了初步的认识。
Objective To be familiar with and master the principle and operation method of Feulgen reaction,meanwhile the morphology and structure of DNA were observed. Methods Take the toad blood cell smear,and hydrolyzing them with 60℃ 1NHCL. Staining cell nuclei with Schiff reagent and counterstaining the cytoplasm with light green. Results Under the microscope,it can be observed that cells nucleus has been stained into purple red and cytoplasm turned into green. Conclusion The nucleus and cytoplasm of cells are represented clearly and distinctively into red and green respectively,which can bring a preliminary acquaintance on biological research to students.
出处
《延安大学学报（医学科学版）》
2015年第4期9-10,共2页
Journal of Yan'an University:Medical Science Edition
