摘要
目的将细胞核DNA倍体分析与高危型人类乳头瘤状病毒检测用于宫颈上皮内瘤变筛查,为提高筛查的准确性探寻一种更有效的方法。方法抽取同时具有宫颈脱落细胞学诊断、高危型人类乳头瘤状病毒检测和组织活检病理诊断结果的209例病例,每例各制备两份宫颈脱落细胞涂片,一份进行细胞学检测,另一份进行宫颈脱落细胞核DNA倍体分析。以组织活检病理诊断结果为"金标准",对细胞核DNA倍体分析和高危型人类乳头瘤状病毒检测结果进行比较。结果年轻组与年老组的高危型HPV感染率的差异有统计学意义;细胞学检测与病理活检结果的一致性不能令人满意;DNA倍体分析与高危型HPV检测结果的差异有统计学意义;以病理活检结果为"金标准",细胞核DNA倍体分析的灵敏度高于高危型人类乳头瘤状病毒检测,但其特异度较低。结论细胞核DNA倍体分析结合高危型人类乳头瘤状病毒检测,可提高宫颈内瘤样变筛查的灵敏性和特异性。
Objective The aim of this study was to compare the value of nuclear DNA ploidy analysis and high-risk human papillomavirus testing for cervical intraepithelial neoplasia screening in order to improve the accuracy of screening. Methods Clinicopathological data of 209 patients who had cervical cytology, high-risk types of human papillomavirus testing and biopsy results were included in this study. Cervical Pap smears were prepared and stained by Feulgen staining for nuclear DNA ploidy analysis. Taking the pathological diagnosis of biopsy as the ＂gold standard＂, the results of nuclear DNA ploidy analysis and high-risk types of human papillomavirus testing were compared and analyzed. Results The difference between the young and elderly groups had statistical significance; The consistency between the results of the Bethesda system and pathological diagnosis of biopsy was weak; The difference between the results of nuclear DNA ploidy analysis and high-risk types of human papillomavirus testing had statistical significance; Taking the pathological diagnosis of biopsy as the ＂gold standard＂, the analysis of nuclear DNA ploidy was more sensitive than high-risk human papillomavirus testing in cervical intraepithelial neoplasia screening, but less specificity than HR-HPV testing. Conclusion The combination of nuclear DNA ploidy analysis and high-risk human papillomavirus testing can enhance the accuracy, sensitivity and specificity of intraepithelial neoplasia detection in cervical screening.
出处
《中国体视学与图像分析》
2010年第2期171-175,共5页
Chinese Journal of Stereology and Image Analysis
基金
广东省医学科学研究基金(A2007094)
