摘要
目的：建立前列回春丸中虎杖苷含量的测定方法. 方法：采用高效液相色谱法测定虎杖苷的含量,色谱柱为Hypersil ODS2 Cl8柱（4. 6 mm ×250 mm,5μm）,检测波长为306 nm,流动相为乙腈-水（V：V=19：81）,柱温为30 ℃,流速为1 ml/min. 结果：虎杖苷质量浓度在1. 97-29. 55 μg/ml范围内与峰面积呈良好的线性关系（r=0. 999 8,n=8）,平均回收率为99. 00%（RSD=1. 28%,n=6）. 结论：该法简便易行、重现性好,可用于前列回春丸中虎杖苷含量的测定.
OBJECTIVE：To establish a method to determine polydatin content in Qianlie Huichun pills. METHODS：The high performance liquid chromatography（ HPLC） was adopted to determine content of polydatin, the chromatographic column was Hypersil ODS2 Cl8 （4. 6 mm× 250 mm,5μm） , the dectation wavelength was 306 nm, the moving phase was acetonitrile-water（V：V=19：81）, the column temperature was 30 ℃ and flow rate was 1 ml/min. RESULTS： The linearity was good（r=0. 999 8,n=8）when the range of polydatin content concentration was from 1. 97 to 29. 55 μg/ml （ r = 0. 999 8 , n = 8 ） . The average recovery was 99. 00% （ RSD = 1. 28%, n = 6 ） . CONCLUSIONS：The method is simple,accurate and reproducible, which can be used to determine polydatin content in Qianlie Huichun pills.
出处
《中国医院用药评价与分析》
2015年第8期1032-1034,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
