摘要
目的观察乌司他丁(UTI)对呼吸机相关性肺损伤(VILI)兔模型血液及肺组织中炎性因子的影响。方法24只家兔随机分为常规潮气量组、大潮气量UTI组和大潮气量对照组,每组8只。常规潮气量组给予潮气量(VT)8 ml/kg通气,大潮气量UTI组给予VT 40 ml/kg通气同时给予UTI干预,大潮气量对照组给予VT 40 ml/kg通气同时给予地塞米松。造模后检测3组静脉血及肺组织白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量,观察动脉血氧分压(Pa O2)、静脉血中性粒细胞计数及肺组织病理变化,检测肺组织中TNF-α、核因子-κB(NF-κB)RNA、蛋白的表达情况。结果与常规潮气量组比较,大潮气量对照组动脉血Pa O2降低,中性粒细胞计数、血清中及肺组织中IL-1β、TNF-α升高,肺组织中TNF-α、核因子-κB(NF-κB)mRNA及蛋白表达均升高(P<0.05);大潮气量对照肺组织病理变化较严重。给予UTI干预后,家兔动脉血中Pa O2有所上升,中性粒细胞计数下降,血清中TNF-α、NF-κB、IL-1β含量及肺组织中TNF-α、NF-κB mRNA及蛋白表达均明显改善(P<0.05);肺组织病理变化减轻。结论 UTI对VILI有保护作用,其机制可能是通过抑制NF-κB的活化,下调TNF-α、IL-1β的表达来实现的。
Objective To observe the effect of Ulinastatin （UTI） on inflammatory factor of blood and lung tissues of rabbit models with ventilator-induced lung injury （VILI）. Methods A total of 24 rabbits were randomly divided into conventional tidal volume （VT） group （ group A, n = 8 ） , high tidal volume and UTI group （ group B, n = 8 ） and high tidal volume control group （ group C, n = 8）. Group A was given VT 8 ml/kg of ventilation, group B was given VT 40 ml/kg of ventilation and UTI intervention at the same time, and group C underwent VT 40 ml/kg of ventilation and Dexamethasone intervention at the same time. After model establishment, interleukin 1 beta （IL-1β） and tumor necrosis factor alpha （TNF-α） contents of venous blood and lung tissues in 3 groups were detected, and arterial oxygen partial pressure （ PaO2 ） , venous blood neutrophil granuiocyte count and pathological changes of lung tissues and lung tissue were observed, and TNF-α, NF kappa B RNA （NF-κB RNA） and protein expression in lung tissues were also detected. Results Compared with those in group A, in group C, the PaO2 level was decreased, neutrophil granulocyte count IL-1β and TNF-ct in serum and lung tissues were increased, and the levels of TNF-α, NF-KB RNA and protein expression in lung tissues were significantly increased （P 〈 0. 05） ; pathological changes of lung tissues in group C were more dramatic. After UTI intervention, PaO2 level in arterial blood of rabbits was increased, the value of neutrophil granulocyte counts was decreased, serum TNF-α, NF-κB and IL-1β, and TNF-α, NF-κB RNA and protein expression in lung tissues were significantly improved （ P 〈 0. 05 ） ; pathological changes of lung tissues were relieved. Conclusion UTI has a protective effect on VILI, and it may be achieved by inhibiting the NF-κB activation and down-regulating the TNF-α and IL-1β expressions.
出处
《解放军医药杂志》
CAS
2016年第1期40-44,共5页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
福州市科技计划项目(2011-S-67-6)
关键词
乌司他丁
呼吸机相关性肺损伤
炎性因子
兔
Ulinastatin
Ventilator-induced lung injury
Inflammatory factor
Rabbits
