摘要
为获得测定大鼠CCI机械痛阈的最佳位置,笔者选取符合条件的SD雄性大鼠,进行坐骨神经慢性挤压性神经损伤模型制作,测定其足底不同位置机械痛阈,并比较其效果。结果为大鼠CCI手术前,1,2,3号位置的痛阈值无显著性差异(P>0.05);CCI手术后,对于手术侧后肢,1,2号位置与3号位置进行比较显示均存在极显著性差异(P<0.001),1号位置与2号位置进行比较无显著性差异(P>0.05);对于未手术侧后肢,1,3号位置与2号位置比较均无显著性差异(P>0.05),但1号位置与3号位置间存在显著性差异(P<0.05)。结果表明,2号位置为CCI模型机械痛阈测定的最佳位置。
Eligible Sprague Dawley （SD） male rats were chosen to make chronic constriction injury （CCI） model of the rat sciatic nerve, and CCI mechanical pain thresholds in different positions of the hind paw of rats were measured and compared to determine the best position for measurement. No significant difference was found in pain threshold among Positions 1,2 and 3 before CCI surgery （ P 〉 0.05 ）, whereas highly significant difference were observed between the Positions 1 or 2 and Position 3 after surgery of hind paw （ P 〈 0. 001 ） and no signifi- cant difference was between Positions 1 and 2 （ P 〉 0.05 ）. For the hind paw prior to surgery no significant difference was observed in pain threshold between the Positions 1 or 3 and the Position 2 in the hind paw, but significant difference were found between the Positions 1 and 2 （P 〈 0.05 ）. The Position 2 is the best place for measurement of mechanical pain threshold in CCI rats.
出处
《热带生物学报》
2015年第4期474-477,共4页
Journal of Tropical Biology
基金
国家自然科学基金(41366002
81420108028)
国家863计划(2012AA021706)
海南省社会发展科技专项(SF201328)
海口市重点科技计划项目(2013-16)
长江学者和创新团队发展计划(IRT_15R15)
海南省普通高等学校研究生创新科研课题(Hys2014-01)
