摘要
采用化学诱变育种手段选育紫薯新品种,分别用0,0.5%,1.0%,1.5%叠氮化钠(Na N3)对亲本澳大利亚Au1990sp紫甘薯胚性细胞团进行诱变处理6 h。结果表明,0.5%NaN_3处理6 h效果最好,将0.5%Na N3处理6 h胚性细胞团进行再分化,共分化出植株125株,2003年移栽后获得99株系,当年入选14株系,秋天收获时其中有3株系高产,编号为CA06产量达到43.98 t·hm^(-2),CA11产量达到40.03 t·hm^(-2),CA17产量达到43.84 t·hm^(-2),对照组Au1990sp产量为4.95 t·hm^(-2)。诱变后的株系在植株表型、特征、特性与亲本相比有很大的不同。对亲本Au1990sp用NaN_3诱变后的株系进行AFLP分析,发现有明显差异,对该品种进行12年的选育,命名为甬紫薯1号,2012年12月获得浙江省非农作物新品种的审定。
In this study, we used chemical mutation breeding method to breed a new variety of purple sweet potato. We adopted 0, 0. 5%, 1.0%, 1.5% of sodiumazide to treat with embryogenic cell masses for 6 hours by using pa- rental Australian purple sweet potato of Au1990sp. The result showed that the treatment of 0. 5% sodiumazide for 6 h was the best. After redifferentiation, the embryogenic callus in the treatment of 0. 5% sodiumazide for 6 h were dif- ferentiated into 125 strains, and 99 strains were obtained after transplantation in 2003. Among the 14 strains selected in 2003, 3 strains obtained high yield, indicating 43.98 t·hm^-2 of CA06, 40.03 t·hm^-2 of CAll and 43.84 t·hm^-2 of CA17. The yield of the control group of Au1990sp was only 4. 95 t·hm^-2. Moreover, the mutated strains showed quite a lot of difference in phenotype, characteristics and features from parental strains. There were obvious differences between the parental strain of Au1990sp and the mutated strain by AFLP analysis. This new variety was named Yongzishu No. 1 after 12 years＇ breeding, which was approved to be a new non-crop variety in December in2012 in Zhejiang Province.
出处
《浙江农业学报》
CSCD
北大核心
2015年第12期2061-2064,共4页
Acta Agriculturae Zhejiangensis
基金
中国农业部948计划(2011-G 31(5))
浙江省重大科技专项农业项目(2012C12902-3)
关键词
甬紫薯1号
诱变育种
品种特性
Yongzishu No. 1
mutation breeding
variety characteristic
