摘要
[目的]建立高效液相色谱-质谱法（HPLC—MS）测定香姜中有机氯农药残留分析方法。[方法]采用正己烷-丙酮（4：1）超声提取，过滤，盐析，干燥，经弗洛里硅土柱净化，用丙酮＋正己烷（10＋90）洗脱，用高效液相色谱-质谱联用法检测香姜中有机氯农药残留。[结果]试验表明，有机氯农药标准的线性范围2～50μg/L，相关系数r均在0．99以上，在5、10、20哕L加标水平下，平均回收率分别为80．0％～95．7％、82．4％～97．2％和83．6％～98．3％：相对标准偏差（RSD）分别为4．2％～9．8％、1．8％～9．4％和2．6％～8．8％。方法检出限（信噪比S／N≥3）均为1μg／kg，方法定量下限（信噪比S／N≥10）均为5μg/kg。[结论]该方法简单、快速、准确，灵敏度和精密度好，适合香姜中有机氯农药残留的测定。
[ Method ] To establish a detection method for the organochlorine pesticides residues in ginger by high liquid chromatograpy-mass spectrometry （HPLC-MS）. [ Method] Ultrasonic extraction was carried out by n-hexane-aeetone （4：1 ）. After filtration, salting out and dr- ying, florida silica column was used for purification; and acetone ＋ n-hexane （10 ＋ 90） was used for elution. Organoehlorine pesticides resi- dues were detected in ginger by HPLC-MS in ginger. [ Result] The standard linear range of organochlorine pesticides was 2 - 50 μg/L; corre- lation coefficient r was above 0.99. The average recovery rates at three levels of 5, 10 and 20 μg/L were 80.0% - 95.7% , 82.4% - 97.2% and 83.6% - 98.3%, respectively. Their relative standard deviations （RSD） were 4.2% - 9.8%, 1.8% - 9.4% and 2.6% - 8.8% , re- spectively. The detection limit （S/N≥3） was 1 μg/kg ; and the quantitation limit （ S/N≥ 10） was 5 μg/kg. [ Conclusion ] This method is simnle, raoid, accurate and sensitive, and is suitable for the determination of organoehlofine nestieides residues in ginger.
出处
《安徽农业科学》
CAS
2016年第2期116-118,共3页
Journal of Anhui Agricultural Sciences
基金
湖南省食品药品监督管理局食品药品安全科技项目(湘食药科R201522)
