摘要
目的探讨波形蛋白(vimentin)和Ki-67在宫颈鳞状细胞癌中的表达及其相关性。方法选择2010年7月至2015年6月入住我院的经病理学诊断为宫颈鳞状细胞癌患者83例作为研究对象,采用免疫组织化学法(SP法)对vimentin及Ki-67在宫颈鳞状细胞癌组织中的表达及相关性进行分析,并探讨上述2种标记物与患者临床特征之间的关系。结果 1vimentin主要表达于间质组织以及部分宫颈癌细胞中,阳性表达主要定位于细胞质。在83例宫颈鳞状细胞癌组织中,63例vimentin表达呈阳性(76%,63/83),平均得分统计量为(2.4±0.6);Ki-67阳性主要定位于细胞核之中,呈棕黄色或者棕褐色颗粒。在83例宫颈鳞状细胞癌组织中,83例Ki-67表达呈阳性(100%,83/83),平均得分统计量为(4.5±0.8);2经Pearson相关性分析,vimentin与Ki-67的表达水平呈正相关(r=0.980,P<0.01);3vimentin及Ki-67蛋白的表达水平与患者是否有淋巴结转移、局部侵袭以及肿瘤细胞分化程度有相关性(P<0.01),但是二者与患者的年龄、肿瘤大小以及临床国际妇产科联合会(FIGO)分期均无相关性。结论 vimentin蛋白与Ki-67在宫颈鳞状细胞癌术后病理诊断中的联合使用,能够判断其生物学行为以及预后状况,从而为宫颈鳞状细胞癌的预防与治疗提供客观依据。
Objective To discuss the expression and their correlation of vimentin and Ki-67 in the cervical squamous cell carcinoma. Methods Eighty-three cases of patients hospitalized from July 2010 to June 2015 in our hospital, who were diagnosed squamous cell carcinoma by pathology diagnosis, were selected as the observation group. The expression of vimentin and Ki-67 was detected by using the immunohistochemical method （SP method） and the relationship between the two markers and the clinical characteristics of the patients were analyzed. Results （1）vimentin was mainly expressed in the interstitial tissue and in some cervical cancer cells. The positive expression was mainly located in cytoplasm. In 83 cases of cervical squamous cell carcinoma tissue, vimentin expression was positive in 63 cases （76%, 63/83）, and the average score was 2.4±0.6; the positive Ki-67 was mainly located in the nucleus, and it showed brownish yellow or brown particles. In 83 cases of cervical squamous cell carci- noma tissue, Ki-67 expression were positive in 83 cases （100% ,83/83）, and the average score was 4.5±0.8. （2） By Pearson correlation analysis, vimentin was positively correlated with the expression level of Ki-67（r=0. 980, P〈0.01）. （3）vimentin and Ki-67 expression had a significant correlation with whether patients had lymph node metastasis, local invasion and tumor cell differentiation degree（P〈0.01）, but there was no correlation with the ages of the patients, tumor sizes and clinical FIGO stages. Conclusion The combination use of vimentin and Ki-67 in pathological diagnosis of postoperative cervical squamous cell carcinoma can improve the judgment of its biological behavior and prognosis, so it can provide the objective basis for the prevention and treatment of cervical squamous cell carcinoma.
出处
《山西医药杂志》
CAS
2016年第1期17-19,共3页
Shanxi Medical Journal
