摘要
目的对广州市医疗机构常用梅毒血清抗体检测试剂功效性进行评价。方法以美国BD公司快速血浆反应素环状卡片试验(RPR)检测试剂和日本富士瑞必欧株式会社的梅毒螺旋体颗粒凝集试验(TPPA)检测试剂作为参比试剂,评估4种非梅毒螺旋体血清抗体和5种梅毒螺旋体血清抗体检测试剂,每种试剂检测梅毒患者血清189例,健康对照30例,比较各试剂的敏感性、特异性和Kappa值。结果 4种非梅毒螺旋体血清抗体检测试剂和5种梅毒螺旋体血清抗体检测试剂与参比试剂相比,敏感性、特异性均大于95%,Kappa值均大于0.90,一致性好。结论广州市医疗机构选用试剂性能较好,试剂评估需每年坚持。
Objective To investigate syphilitic spirochaeta antibody test kit from different manufacturers. Methods The sensitivity, specificity, and Kappa values were compared between 4 kinds of non-syphilitic spirochaeta serum antibody test kit and a reference reagent [ US BD＇s rapid plasma reagin test （RPR） kit] , and 5 kinds of syphilitic spiroehaeta serum antibody test kit and other reference reagent [ Fuji treponema pallidum particle agglutination test （TPPA） kit ]. Results As four kinds of non-syphilitic spirochaeta serum antibody reagents and five kinds of syphilitic spirochaeta serum antibody detection reagent compared to its reference reagent, the sensitivity and specificity were greater than 95% , and Kappa values were greater than 0. 90, which displayed good consistency. Conclusion The study shows that syphilis serologic assays is better. Evaluation of reagents should be done annually.
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2016年第2期204-206,共3页
The Chinese Journal of Dermatovenereology
关键词
梅毒螺旋体
试剂
敏感性
特异性
Syphilitic spirochaeta
Reagent
Sensitivity
Specificity
