摘要
目的探讨干扰素-γ(IFN-γ)多态性及其血清水平与女性生殖道沙眼衣原体感染风险的关系。方法选择河北北方学院附属第一医院妇科2019年1月-2020年1月收治的生殖道沙眼衣原体感染女性120例作为研究对象(研究组),选择同期医院进行体检的健康女性100例作为对照组。针对IFN-γ基因+874位点设计特异性引物,采用聚合酶链式扩增反应(PCR)法检测IFN-γ基因+874位点基因型分布,采用酶联免疫吸附法(ELISA)检测血清IFN-γ、转化生长因子-β(TGF-β)和白细胞介素-10(IL-10)水平。结果研究组与对照组患者IFN-γ基因+874位点基因型理论频数分布与实际分布比较差异无统计学意义(P>0.05),具有群体代表性;研究组IFN-γ基因+874位点AA基因型、A等位基因频率高于对照组,TT基因型、T等位基因频率低于对照组,差异有统计学意义(P=0.008、0.001;0.026、0.001)。校正年龄、孕次、产次等一般资料后,多元Logistic回归分析结果发现,IFN-γ基因+874位点携带AA基因型、A等位基因是沙眼衣原体感染的独立危险因素(P=0.006、0.015)。IFN-γ基因+874位点TT型、AT型和AA型IFN-γ、TGF-β、IL-10水平差异有统计学意义(P<0.001),其中AA型IFN-γ、TGF-β、IL-10水平高于AT型和TT型,差异均有统计学意义(P<0.05)。结论IFN-γ基因+874位点基因突变显著增加本地区女性生殖道沙眼衣原体感染风险,其中AA基因型为易感基因型,A等位基因为易感基因。
Objective To explore the relationship between interferon-γ(IFN-γ)polymorphism and its serum level and the risk of female genital tract Chlamydia trachomatis infection.Methods 120 women with genital tract Chlamydia trachomatis infection in the Department of Gynecology of the First Affiliated Hospital of Hebei North University from January 2019 to January 2020 were selected as the research objects and included them in the study group.100 healthy women who underwent physical examination in the hospital during the same period were selected as the control group.Specific primers were designed for IFN-γgene+874 site,and the genotype distribution of IFN-γgene+874 site was detected by polymerase chain amplification reaction(PCR)method.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum IFN-γ,transforming growth factor-β(TGF-β)and interleukin-10(IL-10)levels.Results There was no significant difference between the theoretical frequency distribution of IFN-γgene+874 locus genotype and the actual distribution between the two groups of patients(P>0.05),which was representative of the population.The frequencies of AA genotype and A allele of the IFN-γgene+874 locus in the study group were significantly higher than thode of the control group,and the frequencies of TT genotype and T allele were significantly lower than those of the control group(P=0.008,0.001;0.026,0.001).General data such as age,pregnancy times,parity,etc.were corrected later;the results of multiple Logistic regression analysis found that IFN-γgene+874 locus carrying AA genotype and A allele were independent risk factors for Chlamydia trachomatis infection(P=0.006,0.015).IFN-γ,TGF-β,IL-10 levels of the TT,AT and AA genotypes of IFN-γgene+874 locus were significantly different(P<0.001);IFN-γ,TGF-β,IL-10 levels of the AA genotype were significantly higher than those of AT and TT genotypes(P<0.05).Conclusions IFN-γgene+874 mutations significantly increased the risk of Chlamydia trachomatis infection in the female reproductive tract in t
作者
施文婷
马会
赵晓霞
郭天智
SHI Wen-ting;MA Hui;ZHAO Xiao-xia;GUO Tian-zhi(Department of Gynecology,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000;Department of Obstetrics,Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,China)
出处
《热带医学杂志》
CAS
2022年第3期351-354,共4页
Journal of Tropical Medicine
基金
四川省高校人文社会科学重点研究基地科研项目(CSYG2018-11)
关键词
干扰素-Γ
基因多态性
沙眼衣原体
易感性
Interferon-γ
Gene polymorphism
Chlamydia trachomatis
Susceptibility
