摘要
目的：探究中华苦荬菜提取物Chinensiolide A（C15H18O4）对肺腺A549细胞、肝癌Ble-7402细胞及结直肠腺癌Lo Vo细胞的抗肿瘤活性。方法：用不同浓度的Chinensiolide A处理体外培养的肺腺癌A549细胞、肝癌Ble-7402细胞及结直肠腺癌Lo Vo细胞24 h后,采用四甲基偶氮唑盐比色法（MTT法）检测Chinensiolide A对该3种细胞生长的影响,计算细胞生长抑制率得出半数抑制浓度（IC50）,并观察形态学变化。结果：在1~50μmol/L浓度范围内,Chinensiolide A对肺腺癌A549细胞、肝癌Ble-7402细胞及结直肠腺癌Lo Vo细胞都有较强的抑制率,且随着浓度的增大,抑制作用越强,IC50分别是17.79μmol/L、25.75μmol/L、13.6μmol/L。形态学结果显示,Chinensiolide A对上述3种肿瘤细胞均有不同程度的杀伤作用,其作用随浓度的增加而增强。结论：中华苦荬菜提取物Chinensiolide A在体外可有效的抑制肺腺癌A549细胞、肝癌Ble-7402细胞及Lo Vo细胞的生长,具有较强的抗肿瘤活性。
Objective：To explore the antitumor activity of extract Chinensiolide A from the Ixeris chinensis on A549 cells,Ble-7402 cells and Lo Vo cells.Method：Chinensiolide A was treated with different concentrations of A549 lung adenocarcinoma cells,Ble-7402 liver cancer cells and Lo Vo cells of colorectal adenocarcinoma after 24 h,the MTT method was used to detect Chinensiolide A influence the three kinds of cell on growth, half inhibitory concentration（IC50） was calculated by cell growth inhibition rate, and the morphological changes were observed.Result：In 1 to 50 μmol/Lconcentration range, Chinensiolide A on lung adenocarcinoma A549 cell, Ble-7402 cells of liver cancer and colorectal adenocarcinoma Lo Vo cells had strong inhibition rate, and with the increase of concentration, the stronger the inhibition,the IC50 was respectively 17.79 μmol/L,25.75 μmol/L and 13.6 μmol/L.Conclusion：Chinensiolide A can effectively inhibit the growth on A549 cells, Ble-7402 cells and Lo Vo cells in vitro,it has stronger antitumor activity.
出处
《中国医学创新》
CAS
2015年第34期109-111,共3页
Medical Innovation of China
基金
教育部大学生创新创业重点项目(201311230001)
