摘要
目的:探讨壁虎粉在体外对急性早幼粒白血病HL-60细胞增殖抑制和诱导凋亡作用。方法:采用不同浓度的壁虎份提取物处理白血病细胞HL-60,四甲基偶氮唑盐(MTT)法观察细胞生长抑制作用,以流式细胞仪检测细胞周期变化和Annexin V/PI双染法检测细胞凋亡,细胞免疫荧光染色法观察细胞凋亡及结构变化。结果:MTT法证实壁虎粉对HL-60细胞具有增殖抑制和杀伤效应,并呈浓度和时间依赖性。流式细胞仪检测细胞周期阻滞于G0/G1期。显微镜下可见HL-60细胞出现典型凋亡细胞形态学改变。结论:壁虎粉在体外可抑制急性早幼粒白血病HL-60细胞增殖,诱导细胞凋亡。
Objective：To investigate anti-proliferation and apoptosis effects of Gecko Extract on HL-60 cells.Methods：By culturing in vitro and treated with various concentrations of Gecko Extract,anti-proliferation effects were assessed with methylthiazolyl tetrazolium（MTT） colorimetric assay.the flow cytometry was used to detect apoptotic rates and cell cycle distribution.and morphology of apoptotic cells was observed under fluorescence microscope.Results：MTT showed that anti-proliferation effect of Gecko Extract on HL-60 cells in a concentration and time dependent manner.the flow cytometry showed that after exposure to drugs cell-cycle was blocked at G0/G1 phase.microscope showed that cell presented some morphologic features of apoptosis.Conclusion：Gecko Extract inhibits proliferation of HL-60 cell and induces apoptosis,suggesting that Gecko Extract might be a potential anticancer drugs.
出处
《中外医学研究》
2012年第11期1-3,共3页
CHINESE AND FOREIGN MEDICAL RESEARCH
