摘要
研制一种用于农药残留现场快速检测的微流控芯片。设计制作的一次性高聚物微流控芯片集成进样、酶抑制反应、显色反应及检测单元,结合自制的手持式光度分析检测装置,可以实现对有机磷、氨基甲酸酯类农药的现场、低成本、快速、准确的检测。结果表明：通过在芯片内部固定存储生化试剂,只需一次进样,7 min内即可实现对克百威和乐果的快速检测,最低检出限分别为0.02、0.6 mg/L,对克百威加标回收率为95.0%~103.3%,制作的芯片在1个月内的稳定性较好。使用微流控农药残留快速检测芯片系统,有望实现全自动检测流程,特别适合于基层非专业人员开展现场、快速、高通量的农药残留筛查。
A novel disposable microfludic chip was developed for rapid detection of pesticide residues in agricultural products, which integrated sampling, enzyme inhibition reaction, color development and detection chamber. By coupling with a laboratory-prepared colorimetric detection device, the chip could provide a simple, easy to use, low-cost and sensitive approach for rapid detection of organophosphate and carbamate pesticides on site. Due to pre-storage of biochemical reagents in the chip, the detection could be achieved within 7 min by just one injection. The limits of detection（LOD） of the method were 0.02 mg/L for carbofuran and 0.6 mg/L for dimethoate, the recoveries of carbofuran from spiked samples were in the range of 95.0% to 103.3%. Furthermore, this microfludic chip had a long storage life of up to 30 days. The microfluidic chip coupled with laboratory-prepared detection device is expected to achieve an automatic detection process, and is particularly suitable for on-site, rapid, and high-throughput screening of pesticide residues by non-professionals.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第2期198-203,共6页
Food Science
基金
嘉兴市公益性应用技术研究计划项目(2014AY21024)
关键词
微流控芯片
农药残留
快速检测
低成本
microfludic chip
pesticide residues
rapid detection
low cost
