摘要
采用微波消解-原子荧光光谱技术对不同粮食中的硒含量的测定方法进行了研究。试验结果表明,微波消解时硝酸和盐酸的体积比在7∶3时,样品消解最为彻底;原子荧光光谱仪在灯电流为80mA、负高压为280V、载气流量为300 ml/min、屏蔽气流量为800 ml/min时,仪器的工作状态最佳;硒的标准工作曲线方程为I=139.44C-31.464,其相关系数为0.999 9;该方法的相对偏差为0.64%,回收率为98.6%～102.4%。实践证明,该方法准确度高,灵敏度好,能准确检测不同粮食的硒含量,为人们选择富硒食品提供了参考。
The method for determination of selenium in different kinds of grains by microwave digestion-AFS was researched. When the volume ratio of nitric acid and hydrochloric acid in the microwave digestion instrument was 7 ： 3, the samples were best digested. The working state of the the atomic fluorescence spectrometer was as follows： the current of lamp was 80 mA, the negative high voltage was 280 V,carrier gas flow was 300 ml/min,shield gas flow was 300 ml/min. The equation of working curve of selenium was I= 139.44C-31. 464, and the correlation coefficient was 0. 999 9. The RSD of this determination was 0.64% ,the recovery rate was 98.6% ～ 102.4%. The method had high accuracy and sensitivity,and could accurately detect the selenium content of different grain,which provided a reference for people choosing selenium enriched food.
出处
《粮食与饲料工业》
CAS
2016年第1期68-71,共4页
Cereal & Feed Industry
关键词
微波消解
原子荧光光谱法
粮食
硒含量
microwave digestion
atomic fluorescence spectrometry
grains
selenium content
