摘要
目的:建立HPLC-ELSD法同时测定消栓通胶囊中三七皂苷R1和人参皂苷Rg_1含量的方法。方法:色谱柱为Phenomenex kromasil C18(150mm×4.6mm,5μm),流动相:乙腈(A)-水(B)梯度洗脱程序:0~10min 90%B,10~15min 75%B,15~20min 50%B,20~25min 50%B,流速为1.0mL·min^(-1)。ELSD参数设定:气体流速为2.3L·min^(-1),漂移管温度为105℃。结果:三七皂苷R1和人参皂苷Rg_1分别在15.28~152.8μg·mL^(-1)(r=0.999 2)和49.62~496.2μg·mL^(-1)(r=0.999 0)范围内呈良好的线性关系,平均回收率分别为96.8%、98.0%,RSD分别为1.7%、2.1%(n=6)。结论:建立HPLC-ELSD法同时测定消栓通胶囊中三七皂苷R1、人参皂苷Rg_1含量方法专属性高,精密度、重现性良好,可用于消栓通胶囊的质量控制。
Objective：To establish an method for simultaneous determination of contents of notoginsenoside R1 and ginsenoside Rgl in Xiaoshuantong capsules. Methods： The HPLC assay for the determination of notoginsenoside R1 and ginsenoside Rgl was per- formed on a Phenomenex kromasil Cc18 column （150mm × 4.6mm, 5μm） with the mobile phase consisting of aeetonitrile-water under gradient elution. The flow rate was 1.0mL ·rainI and the column temperature was room temperature. Evaporative light-scattering （ELSD） detector was used. The air flow rate was 2.3 L · min-1and the detector drift tube temperature was 105℃. Results：Good linearities for notoginsenoside R1 and ginsenoside Rg1 were 15.28～152.8μg ·mL-1（r=0. 999 2） and 49.62～496.2μg · mL-1 （r- 0. 999 0）, respectively. The recoveries of notoginsenoside R1 and ginsenoside Rgl were 96.8% and 98.0%, RSD were 1.7% and 2. 1 % （n= 6）, respectively. Conclusion：The methods are special, accurate, reproducible and can be used as quality control methods for Xiaoshuantong capsules.
出处
《亚太传统医药》
2016年第2期23-26,共4页
Asia-Pacific Traditional Medicine
