摘要
为研究小麦花后外源碳、氮供应对籽粒建成及加工品质的影响,以不同类型春小麦品种"克旱19"和"龙麦33"为材料,在小麦抽穗期分别用2%尿素、5%蔗糖和清水进行叶面喷施,籽粒收获后测定蛋白质组分和支、直链淀粉含量,以及面粉的面筋含量和面团拉伸特性,分析蛋白质品质与淀粉积累,及其与面团加工特性的相互关系。结果表明:蔗糖处理下,2品种的支、直链淀粉平均增加了4.3%和5.5%,而尿素处理的支、直链淀粉含量增加了5.3%和8.6%;蔗糖处理下,"克旱19"和"龙麦33"的清蛋白含量分别较对照增加了14.7%和25.3%,醇溶蛋白含量增加了10.2%和8.1%;而尿素处理的谷蛋白含量显著增加了5.0%和7.3%,醇溶蛋白含量增加了16.0%和10.9%;尿素处理显著降低了不溶蛋白含量,"克旱19"和"龙麦33"分别降低了26.3%和44.5%;尿素处理增加了小麦面筋蛋白组成,从而显著提高了面筋含量,增加了面团抗延阻力和拉伸曲线面积;蔗糖处理主要提高了籽粒清蛋白含量,促进淀粉形成。
The crop populations of two different spring wheat genotypes (Kehan 19 and Longmai 33) were sprayed with 2% of urea, 5% of sucrose, and water at heading stage, respectively, to study the effects of exogenous carbon and nitrogen supply on formation and end-use quality of grain. The contents of amylopectin, amylose and protein fractions of grain, and gluten content and tensile property of dough were determined after grain harvest. Then, the protein quality and starch accumulation, and their relationship with end-use quality were analyzed. The results showed that, compared with the control, the contents of grain amylopectin and amylose of both cuhivars were increased on average by 4.3% and 5.5% under the sucrose treatment, and by 5.3% and 8.6% under the urea treatment, respectively. Moreover, the sucrose treatment in- creased the albumin contents of Kehan 19 and Longmai 33 by 14.7% and 25.3%, and the prola- min contents by 10.2% and 8.1%, respectively; the urea treatment enhanced the glutelin con- tents by 5.0% and 7.3%, and the prolamin contents by 16.0% and 10.9%, respectively. How- ever, the contents of insoluble protein in grains under the urea treatment were decreased by 26.3% and 44.5%, respectively for Kehan 19 and Longmai 33. Our results indicated that urea addition promoted the accumulation of gluten protein, and subsequently increased the content of wet gluten of flour and resistance of dough extension and energy, while sucrose addition improved the contents of starch and albumin in grains.
出处
《生态学杂志》
CAS
CSCD
北大核心
2016年第1期11-16,共6页
Chinese Journal of Ecology
基金
农业部公益性行业专项(201303007)
黑龙江省高校寒地作物品种改良与生理生态重点开放实验室开放课题
校重点学科科研骨干基金(2041100008)资助
关键词
碳氮代谢
蛋白质组分
淀粉
加工品质
carbon and nitrogen metabolism
protein fractions
starch
end-use quality.