摘要
目的：建立测定复方亚麻酸软胶囊中大豆卵磷脂和γ-亚麻酸含量的方法。方法：采用高效液相色谱法测定制剂中大豆卵磷脂的含量：色谱柱为Agilent TC-C18,流动相为甲醇-水（84∶16,V/V）,流速为1.0 ml/min,检测波长为219 nm,柱温为30℃,进样量为10μl;采用气相色谱法测定制剂中γ-亚麻酸的含量：色谱柱为DB WAX柱,进样口温度为210℃,程序升温,载气为氦气,流速为1 ml/min,分流比为1∶30,进样量为0.02μl。结果：L-α-卵磷脂和γ-亚麻酸甲酯检测质量浓度线性范围分别为4.64-16.24μg/ml、0.093 44-0.327 04μg/ml（r=0.9999、0.9998）;精密度、稳定性、重复性试验的RSD〈3%;加样回收率分别为96.79%-99.36%（RSD=0.93%,n=6）、96.63%-99.07%（RSD=1.01%,n=6）。结论：该两种方法操作简便、稳定、重复性好,可用于复方亚麻酸软胶囊中大豆卵磷脂和γ-亚麻酸含量的测定。
OBJECTIVE： To establish a method for the contents determination of phospholipid and γ-linolenic acid in Compound linolenic acid soft capsule. METHODS： HPLC was conducted to determine the content of soybean lecithin; the column was Agilent TC-C18 with mobile phase of methanol-water （84 ： 16, V/V） at a flow rate of 1.0 ml/min, the detection wavelength was 219 nm, the column temperature was 30 ℃, and the injection volume was 10 μl.Gas chromatography was conducted to determine the content of γ-linolenic acid in the preparation; the column was DB WAX, injector temperature was 210 ℃ by temperature programmed, carrier gas was helium at a flow rate of 1 ml/min by split injection （split ratio of 1 ： 30）, and the injection volume was 0.02 μl. RESULTS： The linear range was 4.64-16.24 μg/ml for phospholipid （r=0.999 6） and 0.093 44-0.327 04 μg/ml for γ-linolenic acid methy ester（r=0.999 6） ; RSDs of precision, stability and reproducibility tests were lower than 3% ; recoveries were 97,44%-99.36% （RSD=0.93%, n=6） and 97.22%-99.07% （RSD=1.01%, n=6）, respectively. CONCLUSIONS： The method is simple, stable with good separation, and can be use for the contents determination of phospholipid and γ-linolenic acid in Compound linolenic acid soft capsule.
出处
《中国药房》
CAS
北大核心
2016年第3期412-414,共3页
China Pharmacy
