摘要
为了解四川地区规模化兔场金黄色葡萄球菌(S.aureus)的耐药情况,本研究从四川地区部分规模化兔场共分离鉴定出41株S.aureus,采用纸片扩散法对分离株进行了耐药表型鉴定,同时采用PCR方法对相关耐药基因进行了检测。药敏试验结果显示:分离株对β-内酰胺类、氨基糖苷类、四环素类和大环内脂类药物的耐药率分别为87.80%、97.56%、78.05%和56.10%,对多肽类、喹诺酮类及磺胺类耐药率较低。耐药基因检测结果显示:所有分离株均检测到耐药基因,并且29株分离株具有多重耐药性,其中耐药基因mec A、tet、erm、aac(6')/aph(2'')、ant(4',4'')及aph(3')-III的检出率分别为75.61%、70.73%、46.34%、92.68%、80.49%及53.66%。结合耐药表型与耐药基因结果分析显示兔源S.aureus的耐药表型与耐药基因检出率基本呈正相关。本研究通过检测41株兔源S.aureus对临床常用抗生素的耐药性及相关耐药基因,初步掌握了四川地区规模化兔场S.aureus的耐药情况,为该地区规模化兔场预防及治疗葡萄球菌病临床合理用药提供了依据。
To investigate the prevalence and antibiotic resistance of Staphylococcus aureus isolated from rabbit in Sichuan province, forty-one S.aureus were isolated from rabbits reared farms in partials areas of Sichuan and subjected to detections of antibiotics resistance for phenotype and the resistance genotype of β-lactam antibiotics, aminoglycosides, tetracyclines and macrolide by standard disk diffusion tests and PCR, respectively. The results showed that the antibiotic resistances of the isolates were 87.8% for β-lactam antibiotics, 97.56% for aminoglycosides, 78.05% for tetracylines and 56.10% formacrolides, while the resistance rates to polypeptide, quinolones and sulfonamides were relatively low. Among the 41 isolates of S.aureus, 100% were positive for one or more resistant genes and multiple resistance genes were found in 29 isolates. The 75.61% of the isolates harbored mecA gene, 70.73% harbored tet gene, 46.34% were positive for erm gene and the rates for aac（6′）/aph（2′）, ant（4′, 4″） and aph （39-Ⅲ were 92.68%, 80.49% and 53.66%. According to the results the antibiotic resistance phenotypes of S.aureus isolates were positively associated with the rates of the resistant genes. These data provide a basis for the prevention and treatment of S.aureus in Sichuan province.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2016年第1期45-48,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
四川省科技支撑计划项目(2012NZ0005)
关键词
金黄色葡萄球菌
耐药性
耐药基因
家兔
Staphylococcus aureus
antibiotic resistance phenotype
antibiotic resistance genes
rabbit
