摘要
随着合成生物学的发展,人工合成功能元件在代谢工程领域显示出巨大应用潜力。启动子是调控基因转录水平的重要元件,可以利用人工合成启动子文库对代谢途径进行精细调控,使基因适量表达,实现代谢途径的组合优化。文章综述了人工合成启动子文库的研究进展,评述了不同文库的构建策略,讨论了人工合成启动子文库在代谢工程领域的应用,并展望了人工合成启动子文库的发展方向。
With the development of synthetic biology, design and synthesis of functional elements show great potential in assisting and simplifying the study of metabolic engineering. The fundamental level of transcriptional control takes place at promoter elements that drive gene expression. Synthetic promoter library（SPL） provides promoter with different strength for fine-tuned gene expression. SPL is an important solution to optimal expression of gene and combinatorial optimization of metabolic pathways. This review is mainly focused on the current methods and advances in strategies of SPL. Apart from the description of the developments, we discuss the application of artificial promoter in metabolic engineering and research prospect of promoter engineering in the future.
出处
《微生物学通报》
CAS
CSCD
北大核心
2016年第1期198-204,共7页
Microbiology China
基金
国家973计划项目(No.2011CBA00804
2012CB725203)
国家自然科学基金项目(No.21206112
21390201
21176182)
国家863计划项目(No.2012AA02A702
2012AA022103)
天津市自然科学基金项目(No.15JCQNJC06000)~~
关键词
代谢工程
合成生物学
启动子文库
转录调控
组合优化
Metabolic engineering
Synthetic biology
Promoter library
Transcriptional regulation
Combinatorial optimization
