摘要
易错PCR是目前最简单、有效的一种基因体外随机诱变技术,是农业、工业、生物制药等领域获得优异基因的重要手段。本文综述了易错PCR的基本原理、方法、发展历程,以及该技术的最新研究成果、成功应用领域,并提出了目前存在的问题和可能解决途径。
Error-prone PCR is currently the most simple, efficient method for in vitro mutagenesis, and is one of the important means for achieving excellent genes in agriculture, industry and bio-pharmaceutieals etc. This article summarized the basic principle, technique and developing process of error-prone PCR, and the latest research results, successful applications and developments were stated. The existing problems and possible solutions were also discussed.
出处
《核农学报》
CAS
CSCD
北大核心
2013年第5期607-612,共6页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(30270855)
关键词
易错PCR
随机诱变
研究进展
Error-prone PCR
Random mutation
Research progress
