摘要
目的探讨天麻素对大鼠离体心脏缺血/再灌注(I/R)损伤的保护作用。方法 60只SD雄性大鼠随机分为正常对照组、缺血/再灌注组、天麻素预处理(10、50、100、200μmol/L)组,应用Langendorff离体心脏灌注系统建立心脏I/R损伤模型。除正常对照组外,各组分别进行平衡灌注、全心停灌/再灌处理,从心功能各项指标、心肌酶学、心肌梗死面积(TTC染色)及组织病理学(HE染色)变化4个层面评估天麻素预处理对大鼠离体心脏(I/R)损伤的影响,评价不同浓度天麻素预处理对心脏(I/R)损伤心肌的保护作用。结果与缺血/再灌注组相比,不同浓度天麻素预处理组均可改善缺血/再灌注损伤心功能的各项指标,包括左心室发展压(LVDP)、左室内压上升/下降最大速率(±dp/dtmax)和心率(HR);并降低冠脉流出液中肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)和肌钙蛋白(Trop-I)的活性,有效减少心肌梗死面积,其中以100μmol/L浓度的天麻素效果最显著(P<0.05)。结论天麻素预处理对大鼠离体心脏缺血/再灌注引起的心肌损伤有保护作用,其中以100μmol/L的保护作用最为显著。
Objective To investigate protective effects of the Gastrodine on rat myocardial ischemia/reperfusion （I/R） injury. Methods Sixty SD rats were randomly divided into： control group （control） , ischemia-reperfusion （I/R） group, gastrodin pretreatment （10, 50, 100, 2001,Lmol/L） groups. Applications of Langendorff isolated heart perfusion system was used to buih myocardial ischemia/reperfusion injury model. To evaluate different concentrations of gastrodin in the presence or absence MIRI myocardial protection, the balance perfusion, stopping irrigation 30mira and reperfusion 60min were performed in all the groups except the normal enzymes, and cardiac morphological changes （TTC staining） control group. Cardiac electrophysiology index, myocardial were observed for the effects of the Gastrodin pretreatments on myocardial ischemia-reperfusion injury （MIRI）. Results Gastrodin pretreatment inhibited the left ventricular developed pressure （LVDP） I, R-induced left ventricular pressure rise and fall maximum rate （ ＋ dp/dtmax ）. Gastrodin pretreatment, particularly with the concentration of 100μmol/L gastrodin, also reduced the release of the coronary effluent troponin （Trop-1） , myoglobin （MYO） and many other indicators of myocardial enzyme and minimized the damage of, histopathological changes. There was a clear dose-response relationship. Conclusion Castrodin pretreatment on myocardial injury I/R induced was antagonistic, and may improve ischemia/reperfusion cardiac function, in which antagonism 100μmol/L is the most significant.
出处
《解剖学报》
CAS
CSCD
北大核心
2016年第1期107-112,共6页
Acta Anatomica Sinica
基金
国家自然科学基金(81560050,81460210,81260061)
云南省应用基础研究重点项目(2013FB101)
