摘要
该研究以草莓品种‘红颜’（Fragaria ananassa‘Benihoppe’）果实为试材,从红颜草莓果实中克隆得到1个ABA受体基因FaPYL9,该基因含有1个555bp核苷酸的开放阅读框,编码184个氨基酸序列,含有1个氨基酸保守区域PYR_PYL_RCAR_like。FaPYL9基因在草莓果实发育7个时期的表达量分析表明,随着草莓果实的成熟,FaPYL9基因的表达量迅速升高,并且在果实全红时表达量达到最高;干扰草莓果实中的FaPYL9基因,会延迟草莓果实成熟期3～5d,同时会降低与草莓果实着色相关的FaCHS和FaUFGT基因的表达量,并且果实中的蔗糖含量以及花青素含量也随之降低,ABA含量和果实硬度增加。研究表明,FaPYL9基因在草莓果实成熟发育过程中起重要作用,能促进草莓果实成熟。
This research used strawberry‘Benihoppe＇（Fragaria ananassa ‘Benihoppe＇）as test materials,an ABA receptor gene FaPYL9 was isolated from strawberry fruit,it contains an open reading frame of 555 bp nucleotides,encoding 184 amino acid sequence and contains an amino acid conserved region PYR_PYL_RCAR_like.FaPYL9 gene expression in seven periods of strawberry fruit development showed that with strawberry fruit development FaPYL9 gene expression rapidly increased,and had the highest expression in the fruit full red stage.FaPYL9 gene RNA interference in strawberry fruit can delay the strawberry fruit ripe for 3to 5days.Meanwhile FaPYL9 gene RNA interference to reduce expression of fruit color related genes FaCHSand FaUFGT,sucrose and anthocyanin contents in fruit decreased,ABA content and fruit hardness increased.Results showed that the FaPYL9 gene plays an important role in the strawberry fruit development,FaPYL9 gene can promote strawberry fruit ripening.
出处
《西北植物学报》
CAS
CSCD
北大核心
2015年第12期2379-2384,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
江苏省自然科学基金(BK20131243)
江苏省农业三新工程(SXGC[2014]276
SXGC[2015]311
SXGC[2015]312)
江苏省青蓝工程
