摘要
蛋白磷酸酶2C(protein phosphates 2C,PP2C)是脱落酸(abscisic acid,ABA)信号转导途径中的关键负调控因子,在植物抵御逆境胁迫中发挥重要调控作用。为了探讨花生在细胞组蛋白乙酰化水平改变条件下PP2C家族关键基因的转录情况,从基因组数据库筛选得到8个与拟南芥AtPP2C相似的氨基酸序列,多序列比对分析显示它们均含有PP2C蛋白保守结构域,属于花生PP2C家族蛋白。进化树分析表明,花生PP2C家族蛋白与拟南芥AtPP2C的同源性较高。通过生物信息学分析Ah13G43620的氨基酸序列,发现该片段与野生型花生PP2C37高度同源,将其命名为AhPDCP37(PP2C-like domain-containing protein 37,AhPDCP37)。对鉴定得到的PP2C家族成员在叶片和根部的表达情况进行测定,其中AhPDCP37基因表达丰度最高。花生脱水和盐胁迫处理结果显示,蛋白磷酸酶基因AhPDCP37被诱导上调表达。进一步研究发现,在1μmol/L曲古抑菌素曲古抑菌素A(trichostatin A,TSA)处理下,发现花生中AhPDCP37基因表达情况与细胞中组蛋白乙酰化水平相关。在100μmol/L ABA处理2、8 h后,TSA预处理组叶片中AhPDCP37基因表达较对照组显著受到抑制。本研究为进一步分析蛋白磷酸酶在组蛋白乙酰化调节下参与ABA信号途径的作用机制提供了重要的理论基础。
Protein phosphates 2Cs(PP2Cs)genes are important negative regulator of ABA signaling,which play crucial role in abiotic stress resistance.In order to explore the transcription of key PP2Cs family genes in peanut under the condition of altered cellular histone acetylation level,eight amino acid sequences similar to Arabidopsis AtPP2C were screened from the genome database.Multiple sequence alignment analysis showed that they all contained conserved structural domains of PP2Cs protein and belonged to the peanut PP2Cs family protein.Sequence alignment revealed that AhPP2Cs shared high sequence homology with AtPP2C.Through bioinformatics analysis of the amino acid sequence of Ah13G43620,it was found that this fragment was highly homologous to wild peanut PP2C37,so named AhPDCP37(PP2C-like domain-containing protein 37,AhPDCP37).The expression distribution of PP2Cs family genes in leaves and roots of peanut were determined,and the expression abundance of AhPDCP37gene was the highest.Peanut protein phosphatase AhPDCP37 was up-regulated by dehydration and salt stress.Further study revealed that the expression of AhPDCP37 gene was related to the level of histone acetylation in cell under 1μmol/L TSA treament.At the same time,compared with the control group,TSA pretreatment significantly inhibited the expression of AhPDCP37 gene in leaves at 2 and 8 h.This study provides an important theoretical basic further analysis the mechanism of protein phosphatase participating in ABA signaling pathway under the regulation of histone acetylation.
作者
胡凤
彭莉萍
周涛
喻周运
胡博
苏良辰
Hu Feng;Peng Liping;Zhou Tao;Yu Zhouyun;Hu Bo;Su Liangchen(Department of Biological Engineering,Zhuhai Campus of Zunyi Medical University,Zhuhai,519041;Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Sciences,South China Normal University,Guangzhou,510631)
出处
《分子植物育种》
CAS
北大核心
2022年第12期3847-3854,共8页
Molecular Plant Breeding
基金
国家自然科学基金地区项目(31860310)
广东省科技创新战略专项(2018A030313629)共同资助。
