摘要
目的对蛇床Cnidium monnieri干燥成熟果实中的色原酮类化学成分进行研究,并探讨其对类成骨细胞UMR106增殖的影响。方法采用多种色谱柱技术进行分离纯化,通过波谱分析鉴定化合物结构。对所分离得到的化合物进行类成骨细胞UMR106增殖活性的测试。结果从蛇床子75%乙醇提取物中分离得到10个色原酮类化合物,分别鉴定为cnidimoside A（1）、cnidimol B（2）、前胡色原酮（3）、5,7-二羟基色原酮（4）、5-O-甲基维斯阿米醇（5）、5-O-甲基维斯阿米醇苷（6）、亥茅酚（7）、2,5-二甲基-7-羟基色原酮（8）、升麻素（9）、5-羟基色原酮-7-O-β-葡萄糖苷（10）。在样品溶液浓度为0.10 nmol/L条件下,化合物1、5、9、10对类成骨细胞UMR106的增殖促进率分别是30.23%、31.56%、35.29%和33.36%。结论化合物3～10为首次从蛇床属植物中分离得到。化合物1、5、9、10能一定程度上促进UMR106细胞的增殖。
Objective To study the chomenes from the fruit of Cnidium monnieri and their effects on proliferation of UMR106 cells. Methods The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. The effects of all isolated compounds on proliferation of osteoblast-like UMR106 cells were determined. Results Ten compounds were isolated and identified as cnidimoside A（1）, cnidimol B（2）, peucenin（3）, 5,7-dihydroxychromone（4）, 5-Omethylvisamminol（5）, 4′-O-β-D-glucosyl-5-O-methylvisamminol（6）, hamaudol（7）, 2,5-dimethyl-7-hydroxychromone（8）, cimifugin（9）, and 5-hydroxy-chromone-7-O-β-D-glucoside（10）. Compounds 1, 5, 9, and 10 showed the significant proliferative activities against UMR106 cells lines at the concentration of 0.10 nmol/L and the proliferative ratios were 30.23%, 31.56%, 35.29%, and 33.36%. Conclusion Compounds 3—10 are isolated from species of genus Cnidium Cuss. for the first time. Compounds 1, 5, 9, and 10（0.10 nmol/L） could increase the proliferation of UMR106 cells to some extent.
出处
《中草药》
CAS
CSCD
北大核心
2015年第22期3310-3313,共4页
Chinese Traditional and Herbal Drugs
基金
河北省中医药管理局科研计划项目(2014016)
