摘要
目的探讨二苯并吖庚因(DBZ)对大鼠肺动脉高压的影响。方法将30只大鼠随机分为正常对照组、模型对照组和DBZ组,每组10只。正常对照组:腹腔注射0.9%氯化钠溶液;模型对照组:建立大鼠肺动脉高压模型,腹腔注射0.9%氯化钠溶液5 mg·kg^(-1);DBZ组:建立大鼠肺动脉高压模型,静脉注射DBZ1 mg·m L^(-1)·d^(-1)。超声心动图检测右心室压力,利用苏木精-伊红染色观察肺动脉的重塑,并利用Western Blot法检测肺组织增殖细胞核抗原的表达,CCK-8法检测肺动脉平滑肌细胞的增殖。结果与正常对照组比较,模型对照组大鼠右心室压力增高[(4.60±0.16)k Pa比(3.37±0.18)k Pa],差异有统计学意义(P<0.01),而且肺动脉重塑及肺动脉平滑肌增殖均明显增加。与模型对照组比较,DBZ组大鼠肺组织中右心室压力降低,肺动脉重塑以及肺动脉平滑肌增殖均显著降低。体外实验结果表明,与模型对照组平滑肌细胞比较,低氧诱导后DBZ处理组平滑肌细胞的增殖显著降低[(2.073±0.064)比(4.392±0.013)],差异有统计学意义(P<0.01)。结论 Notch信号通路参与肺动脉高压病理过程,DBZ通过抑制Notch信号通路减少平滑肌细胞增殖,对肺动脉高压大鼠有保护作用。
Objective To investigate the effect and mechanism of dibenzazepines（ DBZ） injection on pulmonary hypertension in rat. Methods Rat models of pulmonary hypertension were established,and 30 male rats were randomly divided into 3 groups： normal control group with saline injection,pulmonary hypertension model control group with hypoxia treatment and saline injection, DBZ group with hypoxia treatment and DBZ injection. The right ventricular pressure was determined by ultrasound cardiogram.Pulmonary arterial remodeling was detected by HE staining.Proliferation cell nuclear antigen and CCK-8 in pulmonary arterial smooth muscle cells were detected by Western blotting. Results The right ventricular pressure of pulmonary hypertension group was significantly increased compared with normal control group [（ 4.60±0.16） k Pa vs.（ 3.37±0.18） k Pa（ P〈0.01） ]. After hypoxia treatment,pulmonary arterial remodeling and proliferation of pulmonary arterial smooth muscle cells of the rats of pulmonary hypertension group were augmented remarkably. Rats from DBZ group showed reductions in right ventricular pressure, amelioration in pulmonary arterial remodeling and suppression in proliferation of pulmonary arterial smooth muscle cells. The proliferation of rat pulmonary artery smooth muscle cells decreased significantly in DBZ treated group [（ 2.073±0.064） vs.（ 4.392±0.013） ] compared with model control group（ P〈0.01）. Conclusion Notch pathway takes part in the process of pulmonary hypertension,and DBZ injection can significantly suppress the proliferation of pulmonary artery smooth muscle cells with a protective effect on pulmonary hypertension.
出处
《医药导报》
CAS
2015年第12期1576-1579,共4页
Herald of Medicine
关键词
二苯并吖庚因
NOTCH信号通路
平滑肌细胞
肺动脉
增殖
细胞
肺动脉高压
Dibenzazepines
Notch pathway
Smooth muscle cells
pulmonary arterial
Proliferation
cell
Pulmonary arterial hypertension
