摘要
目的 Notch在肺动脉高压形成过程中起到重要作用,本文拟探讨可溶性Jagged1(sJag1)对MCT诱导的大鼠肺动脉高压的影响。方法用MCT诱导SD大鼠形成肺动脉高压,分别用载体和sJag1复合体在肺动脉高压诱发开始时加以治疗,测量治疗后肺动脉压和血管中层厚度比例,以及notch1和Jagged1蛋白的表达,并检测血管平滑肌细胞的增生和凋亡情况。结果与载体治疗组相比,sJag1治疗可以通过抑制血管平滑肌细胞增生并促进其凋亡,从而明显缓解肺动脉高压和减轻肺血管中层厚度。结论 sJag1对MCT诱导的大鼠肺动脉高压有明显的抑制作用。
Objective To investigate the effect of soluble Jagged - 1 （ sJagl ） on MCT - induced pulmonary hypertension （PH） in rats. Methods To induce HP in SD rats with MCT, and treat with carrier and sJagl complex at the beginning of PH, then measure the pulmonary artery pressure, vascular media thickness ratio, notchl and Jaggedl protein expression, and proliferation and apoptosis of vas- cular smooth muscle cells. Results Compared with the carrier - treated group, sJagl can significantly relieve pulmonary hypertensionand reduce pulmonary vascular medial thickness by inhibiting vascular smooth muscle cell proliferation and apoptosis. Conclusion sJagl can significantly inhibit MCT- indoeed pulmonary hypertension in rats.
出处
《医学研究杂志》
2013年第10期96-99,共4页
Journal of Medical Research
