摘要
以胡椒属种质蒌叶、墨西哥胡椒、假荜拔为材料,利用顶空固相微萃取-气相色谱和质谱联用(HSSPME-GC/MS)技术测定3种种质叶片中的挥发性成分及用面积归一法计算其相对含量。结果表明:鉴定出蒌叶、墨西哥胡椒、假荜拔的挥发性成分59种,其中从蒌叶中共鉴定出挥发性成分36种,其含量较高的有安息香醛、荜澄茄油烯和β-石竹烯,相对含量分别为23.56%、9.36%和8.54%;从墨西哥胡椒中鉴定出挥发性成分18种,其中含量较高的有肉桂醛、萜品烯和萜品油烯,相对含量分别为77.98%、5.88%和5.11%;而假荜菝叶片挥发性成分有25种,含量较高的有β-石竹烯、罗勒烯和芳樟醇。挥发性成分的种类在3种胡椒属种质中都不相同,总的相对百分含量也不同,说明不同的种质,叶片挥发性成分有一定的差异。
The volatile constituents of black pepper leaves from P. betle, P. auritum, P. retrofractum were analyzed by HS-SPME-GC/MS, and the relative contents were determined by normalization of area. A total of 59 volatile components from three kinds of black pepper were identified. 36 constituents were identified from the leaves of P. betle and the most abundant three were 3-Nitrostyrene, Cubebene and β-Caryophyllene and the relative contents were 23.56%, 9.36% and 8.54%, respectively. Eighteen constituents were identified from the leaves of P. auritum and the most abundant three were Cinnamaldehyde, terpinene and Terpinolene and the relative contents was 77.98%, 5.88% and 5.11%, respectively. The volatile components in P. retrofractum were mainly β-Caryophyllene, Ocimene and Linalool. The types and total relative content of the volatile components in the black pepper were different, which indicating that there were some differences in chemical composition among different varieties. The experiment method of HS-SPME with GC/MS could accurately reflect the volatile components in black pepper. The study may provide a theoretical basis for the further development and utilization of the Piper L.
出处
《热带作物学报》
CSCD
北大核心
2015年第12期2276-2282,共7页
Chinese Journal of Tropical Crops
基金
物种资源保护项目(No.15RZZY-13
2015NWB050)
