摘要
为了明确根癌农杆菌介导的外源蛋白在茄科植物叶片细胞中能否瞬时表达,克隆了土壤根癌农杆菌的Vir E2基因,并融合至绿色荧光蛋白(EGFP)的N端,成功构建p PZP-Vir E2-N-EGFP植物表达载体。以茄科的本氏烟为对照,根癌农杆菌介导在辣椒和番茄叶片细胞中进行了表达分析。用共同聚焦显微镜可以在本氏烟和辣椒叶片细胞中观察到绿色荧光,而在番茄叶片细胞中未观察到。表明外源蛋白Vir E2-N-EGFP可以在辣椒叶片细胞瞬时表达。该研究结果为进一步研究辣椒中与植物病原互作基因的功能奠定了基础。
In order to make it clear that whether heterologous protein could be transiently expressed in Solanaceae plants,we cloned the Vir E2 gene from agrobacterium by PCR,fused it to the N terminal of EGFP and successfully constructed the plant expression vector p PZP-Vir E2-NEGFP. Vir E2-N-EGFP was expressed in the leaf cells of Capsicum annuum and Lycopersicon esculentum Mill mediated by agrobacterium,with the Nicotiana benthamiana as control. Green fluorescence was observed in the leaf cells of Nicotiana benthamiana and Capsicum annuum,but not in that of Lycopersicon esculentum Mill. The results indicated that the heterologous protein Vir E2-N-EGFP could be successfully expressed in the leaf cells of Capsicum annuum,which provide a basis for research on the function of the pathogeny interacted protein in Capsicum annuum.
出处
《安徽农业科学》
CAS
2015年第33期254-256,265,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30900937)
中央高校基本科研业务费专项资金项目(XDJK2016A009
2362015xk04)
中国烟草总公司四川省公司科技项目(201202007)
中国烟草总公司湖南省公司科技项目(14-16ZDAa02)
