摘要
细胞的增殖、分化和凋亡等生物学行为受其所处的微环境调控,而微环境包含剪切力和生化因子等理化因素。为离体研究胞外微环境剪切力和生化因子对细胞的联合作用,该文基于流体力学和物质传输的原理设计了一种包括生化因子入口端A和非生化因子入口端B的Y型微流控装置。通过保持入口端A的体积流量率恒定,动态调控入口端B的体积流量率,可在混合通道内提供单纯的动态剪切力、动态剪切力和动态生化因子、以及动态剪切力和稳定的生化因子三种刺激。对混合通道中的非定常层流和Taylor-Aris弥散方程进行数值求解,获得了混合通道内动态剪切力和生化因子浓度的分布,随后利用荧光粉溶液进行了实验验证。数值仿真和实验验证结果均显示该文设计的微流控装置为进一步研究离体培养细胞在剪切力和生化因子联合刺激下的生物学效应提供了可能性。
The cellular biological behavior, such as proliferation, differentiation and apoptosis, is regulated by the cellular niche. The physical and chemical factors, such as fluid shear stress and biochemical factors, are included in the cellular microenvironment. In order to investigate the cultured cells in vitro in response to the combined effects of shear stress and biochemical factors in cellular microenvironment, a Y-type microfluidic device which has an inlet A with biochemical substance and an inlet B without biochemical substance is designed based upon the principles of fluid mechanics and mass transfer. Three different types of stimuli, including dynamic shear stress alone, dynamic shear stress and dynamic biochemical factor, as well as dynamic shear stress and static biochemical factor, could be provided in the mixing channel by keeping a constant volumetric flow rate at the inlet A and dynamically changing the volumetric flow rate at the inlet B. The dynamic shear stress and the concentration profiles of biochemical factors are obtained by numerically solving the equations governing the unsteady laminar flow and Taylor-Aris dispersion. The experimental validation is also presented by using fluorescent solution. Both the results from numerical simulations and experimental studies have shown the microfluidic device designed in this paper could provide a possibility for further investigating the biological cells cultured in vitro in response to combined effects of shear stress and biochemical factors.
出处
《水动力学研究与进展（A辑）》
CSCD
北大核心
2015年第6期643-649,共7页
Chinese Journal of Hydrodynamics
基金
国家自然科学基金(11172060
31370948)
辽宁省自然科学基金(2015020303)~~
关键词
微流控装置
剪切力
动态生化因子
生物细胞
联合作用
microfluidic device
shear stress
dynamic biochemical factor
biological cell
combined effect
