摘要
目的建立一种高效液相色谱法（high performance liquid chromatography,HPLC）,用来测定人头发中的喹硫平浓度。方法色谱柱使用Inertsil ODS-C18柱（4.6 mm×150 mm,5μm）,流动相为0.01 mol/L醋酸铵-甲醇（32︰68）,流速为1.0 m L/min,柱温40℃,检测波长为254 nm,以正已烷为萃取剂,建立HPLC法。结果喹硫平在5-200 ng/mg范围内峰面积与浓度呈良好线性关系,萃取回收率〉75.0%,方法回收率在95.0%-105.0%之间,日内和日间精密度均〈10.0%,符合生物样品分析要求。结论本研究建立了测定人头发中喹硫平浓度的HPLC法,该方法操作简单,结果灵敏、准确,具有一定的应用价值。
Objective To establish a method for determinning quetiapine in human hair by HPLC. Methods A reverse phase HPLC system was performed on Inertsil ODS-C18column（ 4. 6 mm × 150 mm,5 μm） with the mobile phase consisted of 0. 01 mol / L ammonioum-methanol（ 32 ︰ 68）. The flow rate was 1. 0 m L / min,column temperature was 40 ℃,the detection wavelength was 254 nm. N-hexane was used as extracting solvent. Results The calibration curve was linear in the range of 5-200 ng / mg. for quetiapine. The recovery of extraction 75. 0%,the recovery of method was between95. 0% and 105. 0%,the intra-day and inter-day precision were all no more than 10. 0%. This method met the requirements of biological samples.Conclusion A HPLC method of concentration of quetiapine in human hair is established,which is simple,sensitive,accurate and has a certain value.
出处
《中国生化药物杂志》
CAS
2015年第9期161-163,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
南京医科大学科技发展基金(2013NJMU207)
关键词
头发
喹硫平
高效液相色谱
hair
quetiapine
high performance liquid chromatography
