摘要
建立指甲中氯氮平的液相色谱-串联质谱测定方法,并考察长期用药的精神疾病患者指甲中氯氮平的存在情况。指甲经冷冻研磨处理后,利用乙腈-20 mmol.L1乙酸铵缓冲液(7∶3)超声提取。采用Ultra IBD柱分离,二级质谱多反应监测模式(MRM)检测。指甲中氯氮平在0.5～50 ng.mg1内线性良好(r>0.999 9),日内和日间精密度均小于7%,准确度范围为97.4%～99.6%。16名长期用药的精神疾病患者指甲中氯氮平浓度为1.60～14.14 ng.mg1;其去甲代谢物亦被检出。指甲中氯氮平浓度与毛发存在一定相关性(r2=0.971),并略低于同源个体的毛发中氯氮平浓度。
As a keratinized material, nail recently has attracting researchers＇ attention in the pharmaceuticals analysis, There are comparatively limited studies concerning nail＇s xenobiotic determination and its mechanism This article reported the development of a sensitive, specific and reproducible LC-MS/MS method, which could be as a foundation of other studies on drug determination in nail. It can also be regarded as the first report on organic drug in China's Mainland. Sixteen nail samples from volunteers, who were ingested clozapine for more than nine months, are confirmed positive after being analyzed by the method. It is found that contents of clozapine in the patients＇ nails are above the nanogram level. Besides, a comparative study of clozapine concentration in nails and hair was made, with a result that there exists a correlation between the two materials in terms of clozapine concentration.
出处
《药学学报》
CAS
CSCD
北大核心
2012年第9期1193-1199,共7页
Acta Pharmaceutica Sinica
基金
Project supported by National Natural Science Foundation(20975070)
the Shanghai Key Forensic Laboratory Funded Project(12DZ2271500)
