摘要
目的探讨大黄素对小鼠免疫功能的影响及其溶血毒性。方法制备并体外培养小鼠特异性免疫细胞T、B淋巴细胞及非特异性免疫细胞巨噬细胞、NK细胞,采用浓度为5、10、15、20μM的大黄素分别处理各免疫细胞,另设DMSO为对照组,采用中性红法和MTT法检测大黄素对免疫细胞功能的影响。采用浓度为20、40、60、80μM的大黄素进行体外溶血实验,以生理盐水为空白对照组,以无菌蒸馏水为阳性对照组,观察大黄素的溶血毒性。结果与对照组比较,5、10、15、20μM组对T、B淋巴细胞增殖的影响差异均不具有统计学意义（F=0.009,P=1.000;F=0.003,P=1.000）,各剂量组提高巨噬细胞吞噬能力且呈浓度依赖性（F=665.525,P=0.000）,提高巨噬细胞增殖率且呈浓度依赖性（F=134.812,P=0.000）,提高NK细胞活性且呈浓度依赖性（F=200.190,P=0.000）。溶血实验结果显示,大黄素在20~80μM浓度范围内,溶血率测定结果均小于5%。结论大黄素能显著促进小鼠非特异性免疫细胞的活性,在大黄素发挥有效活性的浓度范围内,对红细胞无溶血毒性。
Objective To investigate effect of emodin on mice immune function and its hemolysis toxicity. Methods The mouse specific immune cells of T,B lymphocytes and nonspecific immune cell of macrophages and NK cells were prepared and incubated in vitro. The different immune cells were treated by emodin with different concentrations of 5,10,15 and 20 μM,and DMSO as control group. The effect of emodin on immune cells function was detected by neutral red assay and MTT assay. The hemolysis test in vitro was conducted by emodin with different concentrations of 20,40,60 and 80μM,physiological saline as blank control group and sterile distilled water as positive control group,then the hemolysis toxicity of emodin was observed.Results There were no significant difference of T and B lymphocyte proliferation among control group,5,10,15 and 20 μM group（ F = 0. 009,P =1. 000; F = 0. 003,P = 1. 000）,the phagocytic ability of macrophages enhanced in each dose group and was concentration dependent（ F = 665. 525,P =0. 000）,the proliferation rate of macrophages enhanced and was concentration dependent（ F = 134. 812,P = 0. 000）,the activity of NK cells enhanced and was concentration dependent（ F = 200. 190,P = 0. 000）. Hemolysis test results showed the hemolysis rate was less than 5% in the range of 20 to 80μM emodin. Conclusion Emodin could significantly promote the nonspecific immune cells activity. Within the concentration of experiment,emodin has no hemolysis toxicity.
出处
《中国生化药物杂志》
CAS
2015年第9期34-37,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
辽宁省教育厅科学研究一般项目(L2013412)
大连市科技计划项目(2013E13SF108)
