摘要
于1997年10－11月，以青岛市红岛养殖场养殖的日本对虾为材料，采用分光光度法对其血淋巴中的溶血素进行活性检测，研究多种理化因子对其溶血性能的影响，以及日本对虾溶血素经细菌和虫草多糖肌肉注射刺激后的活性变化。结果表明，日本对虾溶血素的活性同对虾的健康状况密切相关。该溶血累经60℃以下处理溶血作用有所增强，在pH=5－9时溶血活性最高，Ca2＋、Fe2＋可增强溶血作用。注射大肠杆菌（2×107cells／尾）和虫草多糖能够诱导日本对虾溶血素的产生，使溶血活性有所提高；而注射哈维氏弧菌（7×107cells／尾）会导致对虾发病，降低溶血素的活性。
Hemolymph was collected by heart puncture from cultured penaeus japonicus with a body length of 14-16cm from Hongdao prawn culturing fiwn in Qingdao from October to November,1997. Hemolytic activity of heinolymph against chicken red blood cells was detected by colorimetric analysis. The effects of some physical and chemical factors such as heat, pH value, divalent metal cations on the hemolytic activity were studied. Having been stimulated by intramuscular injection with Escherichia coli and Vibrio harveyi, the prawns were nursed in the glass tanks in laboratory.Hemolymph was collected every 24h and changes jn hemolytic activity were detected. Cordycepic polysaccharide extracted from Cordyceps miliaris was used as immunopotentiator. 1.0% polysaccharide was injected intramusculary into the prawns. The hemolymph was collected every 24h for the study of the indued change in hemolytic activity. The results are described below.Th hemolymph of p japonicus possesses hemolytic activity agajnst the red blood cells of chicken. Hemolysin acts as an important component in the immune system of P.japonicus. The activity of hemolysin is closely related to the health status of P.japonicus. There is a significant drop in the hemolytic activity of moribund prawn. Hence, the hemolyhc activity could be used as a prawn's health index.The activity of hemolysin could be improved to some extent undr the treatment at 60℃for 20min. When the temperature went up over 700℃, the hemolymph began to denafor and solidify.The pH range of hemolytic action was from 5 to 9. Hemolytic astivity was also enhanced by Ca2+and Fe2+, but not by Mg2+, Cu2+, Zn2+, Mi2+.Injection with 2 ｘ 10 7 bacteria E. coli could induce the production of hemolysin in the hemolymph of p. japonicus. The increase in hemolysin would be favorable to sterilization and elimination of bactcria in the hemolymph, so as to protect the prawn against the disease. However,with injechon of 7 ｘ10 7 baCteria V. harveyi, the activity of hemolysin of p japonicus increased a littie on
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1999年第4期362-367,共6页
Oceanologia Et Limnologia Sinica
基金
国家攀登计划B资助!PDB-6-6-3
