摘要
以采自汕头的南美白对虾为研究对象,运用病原菌人工感染、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、基质辅助激光解吸电离飞行时间质谱分析(MALDI-TOF/MS)、生物信息学和免疫印迹(Western-blot ting)等现代分子生物学方法,探索和鉴定对虾感染后肝胰脏中的主要变化蛋白。结果表明,南美白对虾人工感染溶藻酸弧菌6h之后,实验组和对照组肝胰脏的SDS-PAGE图谱出现12条明显差异条带,其中分子量分别为75kDa(命名为p75)和73kDa(命名为p73)的两种蛋白变化最为明显,进一步研究显示,p75和p73分别为血蓝蛋白的两个不同的亚基。提示,肝胰脏中的血蓝蛋白可能在对虾抗感染免疫中发挥重要的作用。
A preliminary study was made to identify two types of main differential proteins in the hepatopancreas in whiteleg shrimp Litopenaeus vannamei infected by V. alginolyticus collected from Shantou by artificial infection, MALDI-TOF/MS, bioinformatics and Western-blotting. The results showed that there existed twelve variable bands in SDS-PAGE map in the hepatopancreas between control group and treatment group 6 h after infection. Two proteins with 75 kDa (p75) and 73 kDa (p73) were observed and both p75 and p73 were identified to be two different subunits of hemocyanin, suggesting that the hemocyanin in hepatopancreas may play a significant role in the course of shrimp immune of resisting bacterial infection.
出处
《水产科学》
CAS
北大核心
2005年第6期19-23,共5页
Fisheries Science
基金
广东省自然科学基金(博士启动基金)资助项目(130-934088)
厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室资助项目(2004101).
