摘要
【目的】通过对金娃娃萱草（Hemerocallis hybridus‘Stella de Oro’）在重金属Cd胁迫条件下的生长及生理特性研究,探讨金娃娃萱草抗重金属Cd的能力,为重金属污染土壤的植物修复提供理论依据。【方法】以多年生金娃娃萱草分株苗为材料,利用盆栽试验,以土壤中未施入Cd为对照,探讨土壤中不同Cd含量（0.3,1,20,100mg/kg）和胁迫时间（30,60,90,120d）处理后,金娃娃萱草株高、干质量、叶片光合及抗氧化酶活性等指标的变化。【结果】Cd含量小于1mg/kg时可促进金娃娃萱草生长,Cd含量大于1mg/kg时,植株高度和生物量呈下降趋势;金娃娃萱草叶片叶绿素a和b、总叶绿素、类胡萝卜素含量随Cd含量的增加呈先增后降趋势,转折点出现在1mg/kg处理水平上;与对照相比,金娃娃萱草POD活性随Cd含量的增加逐渐增大;低Cd含量（≤1mg/kg）处理下SOD活性有所增加,尔后随含量的增加SOD活性下降;金娃娃萱草净光合速率（Pn）、蒸腾速率（Tr）和气孔导度（Gs）日变化均呈双峰曲线,峰值出现在10：00和16：00,胞间CO2浓度（Ci）则在10：00和16：00出现谷值。【结论】在Cd（0.3-100mg/kg）含量胁迫的伤害阈值内金娃娃萱草无死亡现象,其适用于含量〈1mg/kg的Cd污染土壤的修复。
【Objective】The study analyzed the heavy metal resistance of Hemerocallis hybridus ‘Stella de Oro＇by investigating its growth and physiological characteristics under Cd stress to provide basis for phytoremediation of heavy metal contaminated soils.【Method】Perennial H.hybridus ‘Stella de Oro＇divided seedlings were planted in pot to investigate the effect of Cd concentration（0.3,1,20,100mg/kg）and times of stress（30,60,90,120d）on plant height,dry mass,leaf,photosynthesis and anti-oxidase activity of H.hybridus ‘Stella de Oro＇plants.【Result】Cd concentrations of less than 1 mg/kg stimulated the growth of H.hybridus ‘Stella de Oro＇,while Cd concentrations of greater than 1 mg/kg decreased the height and biomass of plants.Chlorophyll a,chlorophyll b,total chlorophyll,and carotenoids decreased after the initial increase when mass fraction of Cd increased and the turning point was 1mg/kg.Compared with the CK,POD activity gradually increased with the increase of Cd concentration,while SOD activity was increased slightly followed by decrease when Cd concentration increased.Photosynthetic rate（Pn）,transpiration rate（Tr）,and stomatal conductance（Gs）showed bimodal curves with peaks at 10：00am and 16：00pm,CO2 concentration in the intercellular（Ci）at 10：00am and 16：00pm when the valley value.【Conclusion】Cd（0.3-100mg/kg）stress within threshold caused no death to H.hybridus‘Stella de Oro＇,so it was suitable for remediation of soils with less than 1mg/kg Cd.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2015年第12期174-180,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
吉林省科学技术厅项目"长白山特种经济植物保护及配套关键技术研究"(20100259)
关键词
金娃娃萱草
CD胁迫
土壤修复
Hemerocallis hybridus ‘Stella de Oro＇
Cd stress
soil remediation
