摘要
将鸡传染性法氏囊病免疫复合物疫苗和BX株活疫苗分别免疫1日龄中等母源抗体水平京红雏鸡,同时免疫1日龄SPF雏鸡作对照,免疫后第9天观察法氏囊病变,免疫后第28天采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后第9天,免疫复合物疫苗组SPF雏鸡和京红雏鸡法氏囊均正常,BX株活疫苗组SPF雏鸡法氏囊出现了萎缩病变;免疫后第28天,对照SPF雏鸡和京红雏鸡的免疫复合物疫苗组、BX活疫苗组IBDV中和抗体效价分别为9.2log2、8.5log2和7.2log2、4.4log2,攻毒保护率均为100%。试验结果表明免疫复合物疫苗免疫1日龄中等水平母源抗体雏鸡效果很好,能产生较高的中和抗体,攻毒保护率能达到100%。
One-day-old moderate maternal antibody Jinghong chickens and SPF chickens were vaccinated with infectious bursal disease（IBD）immune complex（IC）vaccine and BX strain live vaccine,respectively,pathological changes of bursa of fabricius in chickens were observed at the 9th day after immunization.On the 28 th day after immunization,blood samples were taken and the IBDV neutralizing antibody were tested,meanwhile experimental chickens were challenged with high virulent IBDV,the protective rates of experimental groups were calculated.The results showed that at the 9th day after immunization,the bursa of fabricius in SPF and Jinghong chickens were normal in IC vaccine group,however atrophic changes of bursa of fabricius in BX strain live vaccine group were shown.At the 28 th day after immunization,the IBDV neutralizing antibody of IC vaccine group and BX strain live vaccine group in SPF and Jinghong chickens were9.2log2,8.5log2,7.2log2 and 4.4log2,respectively,while 100% protection rates were provided.The results provided that IC vaccine had better immunity effect on one-day-old moderate maternalantibody chicken,high levels of neutralizing antibodies against IBDV were produced and protection rates were 100%.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第12期3338-3343,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31402230)
北京市自然科学基金(6132014)
北京市农林科学院创新能力建设项目(KJCX20140413)
关键词
鸡传染性法氏囊病
免疫复合物疫苗
法氏囊
保护率
infectious bursal disease（IBD）
immune complex vaccine
bursa of fabricius
protection rate
