摘要
目的研究榄香烯对肺癌A549细胞的抑制作用及分子机制。方法取肺腺癌细胞株A549,分别以10μg/m L、20μg/m L、40μg/m L、80μg/m L、160μg/m L榄香烯处理,标记为观察组1-5,并设空白对照组6。于处理24 h、48 h、72 h后,以流式细胞仪检测细胞周期及细胞凋亡率,以Wsetern Bolt法检测真核起始因子4E（e IF4E）蛋白水平。结果 1处理24 h后,各组细胞周期无明显变化,G2/M期、sub-G1期细胞比例差异无统计学意义（P〉0.05）;处理48 h、72 h后,观察组G2/M期细胞比率较对照组显著性下降,差异有统计学意义（P〈0.05）,但观察组组内时点比较,差异无统计学意义（P〉0.05）;榄香烯浓度越高,抑制作用越明显。2随药物浓度增长,细胞凋亡率显著性提升。3相较对照组,处理24 h后,观察组e IF4E表达呈下降趋势,且浓度越高,表达越低。结论榄香烯对肺癌A549细胞抑制作用强,其对细胞周期的影响存在剂量与时间依耐性;细胞凋亡率随药物浓度增加而增加,但过高剂量可能导致毒副作用,降低凋亡率;对A549细胞增殖抑制的生理机制可能与抑制e IF4E表达有关。
Objective To study the inhibitory effect and molecular mechanism of elemene on A549 lung cancer cells. Methods Lung adenocarcinoma cancer cells A549 were treated with elemene of 10 mu g / m L,20 mu g / m L,40 mu g / m L,80 mu g / m L,160 mu g / m L,marked as observation group 1 - 5,with a blank control group 6 set up. The cell cycle and cell apoptosis rate were detected with flow cytometry at 24 h,48 h,72 h after elemene treatment,and e IF4 E protein levels by Wsetern Bolt method. Results（ 1） At 24 h after treatment,there was no obvious change in each cell cycle,and the difference in cell percentage in G2/ M phase,the sub- G1 phase was of no statistical significance（ P 〉 0. 05）; and at 48 h,72 h after treatment,the cell ratio in observation group during G2/ M phase was significantly decreased compared with control group,and the difference between the two was statistically significant（ P 〈 0. 05）,but compared within the observation point,there was no statistical difference（ P 〉 0. 05）; The higher the concentration of elemene was,the more obvious was its inhibitory effect.（ 2） With the growth of drug concentration,the cell apoptosis rate was markedly elevated.（ 3） In comparison with control group,24 h after treatment,e IF4 E expression in observation group was on the decline. And the higher the concentration was,the lower was the expression. Conclusion The inhibitory effect of elemene on A549 lung cancer cell is strong,and its influence on cell cycle is of dependency on dose and time; Cell apoptosis rate increased with the increase of drug concentration,but higher doses can cause side effects,reduce the apoptosisrate; physiological mechanism of A549 cell proliferation inhibition may be related to expression of inhibiting e IF4 E.
出处
《上海预防医学》
CAS
2015年第11期690-693,共4页
Shanghai Journal of Preventive Medicine
关键词
榄香烯
肺癌A549细胞
细胞凋亡
分子机制
Elemene
Lung cancer cells of A549
Cell apoptosis
Molecular mechanisms
