摘要
目的观察听力正常豚鼠通过掩蔽能否引出声诱发短潜伏期负电位（acousticallyevokedshonlatencynegativeresponse，ASNR），并与药物致聋豚鼠的ASNR进行对比，同时验证二者的神经核团来源是否相同。方法40只健康豚鼠采用随机数字表法分为三组：对照组8只（16耳）、掩蔽组16只（32耳）和致聋组16只（32耳）。掩蔽组选择气导白噪声作为掩蔽声，采用15dB的掩蔽／刺激强度差以避免掩蔽不足和掩蔽过度，根据ASNR引出与否，分为掩蔽ASNR组和掩蔽非ASNR组。致聋组采用卡那霉素和利尿酸联合致聋，根据ASNR引出与否，分为致聋ASNR组和致聋非ASNR组。全部豚鼠均行前庭神经核定位及直流电损毁，24h后再次行听性脑干反应（ABR）／ASNR测试，比较损毁前后ABR／ASNR的改变情况。脑干切片显微镜下验证电解损毁部位。结果掩蔽组32耳中24耳引出ASNR，致聋组26耳重度聋耳中12耳引出ASNR，掩蔽组ASNR的引出率（75．0％）高于致聋组（46．2％），差异具有统计学意义（x2=5．07，P=0．024）；两组引出的ASNR在阈值和潜伏期上差异均无统计学意义（P值均〉0．05）。正常对照组前庭神经核损毁后，ABR波形及引出率无明显改变。掩蔽ASNR组和致聋ASNR组引出耳前庭神经核损毁后，ASNR均消失。脑干切片显示电损毁前庭神经核部位均准确无误。结论利用适当强度的白噪声掩蔽可以成功地在豚鼠听力正常耳引出ASNR，其波形、阈值和潜伏期与药物致聋豚鼠重度听力损失耳引出的ASNR相同，二者均起源于前庭神经核。
Objective To compare acoustically evoked short latency negative responses （ASNR） elicited from normal and profound hearing loss guinea pig ears and to confirm their vestibular nuclei origination. Methods Forty healthy guinea pigs were employed in the experiment, which were randomly divided into the control group （8 subjects, 16 ears）, the masking group （16 subjects, 32 ears） and the deafened group （16 subjects, 32 ears）. Air conductive white noise was chosen for masking. Masking dilemma was avoidable by an appropriate 15 dB gap between stimulus and masking sound. Both the masking group and the deafened group were further divided into ASNR group and non-ASNR group based on the presence of ASNR. Electrolytic lesion was conducted to the vestibular nuclei, followed by ABR/ASNR recording. The lesioned brainstem slices were microscopically verified. Results In the masking group, ASNR were present in 24 ears （75.0% , 24/32） and 12 ears （46. 2% , 12/26） in deafened group, showing statistically higher presence rate for masking group （ X2 = 5.07, P = 0. 024 ） . There were no significant differences for the ASNR threshold and latency between the masking ASNR group and the deafened ASNR group. For the two ASNR groups, electrolytic destruction to the vestibular nuclei subsequently eliminated the ASNR. Braiustem slice proved the accurate sites of electrolytic lesion. Conclusion Moderate white noisemasking prevents hearing system potential overlap without affecting vestibular system, therefore, ASNR is successfully elicited in normal guinea pigs. Both ASNRs from normal and deafened guinea pigs are of similar natures and origination from, the vestibular nuclei.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2015年第12期1015-1019,共5页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
国家自然科学基金（30360110）
广西科学基金（桂科回0342010）
2014年广西研究生教育创新计划资助项目（YCSZ2014113）
关键词
诱发电位
听觉
脑干
前庭神经核
知觉掩蔽
豚鼠
Evoked potentials, auditory, brain stem
Vestibular nucleus
Perceptual masking
Guinea pigs
