摘要
目的:探究薛凡尼氏炭角菌菌丝体液体发酵培养的最佳营养条件和最适环境条件及人工培养子实体使用固体培养料的最适配方。方法:以菌丝体生物量为指标,采用单因素实验研究不同碳源、氮源、无机盐、温度、p H、装液量对薛凡尼氏炭角菌菌丝体液体发酵培养的影响;以子实体生物学效率为指标判断不同配方固体培养料对人工培养子实体的影响。结果表明:乳糖为最优碳源,蛋白胨为最优氮源,供试无机盐硫酸锰、磷酸二氢钾、硫酸镁、硫酸钾对菌丝体生长有一定的促进作用;最适培养温度为30℃,发酵液最优的p H为7,最适的装液量为50 m L/250 m L;用68%木屑加30%麸皮的固体配方为薛凡尼氏炭角菌子实体培养的最优配方,生物学效率达到8.14%(w/w,dry)。结论:此法适用于薛凡尼氏炭角菌菌丝体的液体培养,并首次人工培养出薛凡尼氏炭角菌子实体。
Objective：Based on experiments,this research attempted to find out the optimal nutritional conditions,optimal growth environment for Xylaria schweinitzii mycelium. It also explored the best formula of solid substrate for its cultured fruiting body. Methods ： Using mycelium biomass as the indicator, single- factor experiments were conducted in an effort to study the effects of different carbon sources,nitrogen sources,mineral salts,temperature,p H and liquid volume used in the solid substrate on the growth of cultured fruiting body Xylaria schweinitzii. Biological efficiency was used to measure the effects of different formula of solid substrate on cultured fruiting bodies of Xylaria schweinitzii. Results：Results showed that lactose was the optimum carbon source,and peptone was the optimum nitrogen source,and mineral salt which was Mn SO4,KH2PO4,Mg SO4,K2SO4 had positive effects in promoting the growth of mycelium. It was also found out that the optimal temperature was 30 ℃,the optimum p H for fermentation was 7,the optimum liquid volume was 50 m L and optimal solid substrate consists of 68% sawdust and 30% bran. When the optimal formula was used,the biological efficiency reached 8.14%（w/w,dry）. Conclusion：This research tried out a liquid culture method for culturing Xylaria schweinitzii. And for the first time,this method was successfully used to culture fruiting bodies of Xylaria schweinitzii.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第24期190-193,共4页
Science and Technology of Food Industry
基金
国家自然科学基金(21272189)
