摘要
目的对手足口病(hand-foot-mouth disease,HFMD)进行病原学监测,了解北京市西城区手足口病的流行特征。方法提取标本中病毒核酸,采用实时荧光PCR法进行核酸鉴定及分型。结果 2012年-2014年共收集咽拭子标本882份,肠道病毒(enterovirus,EV)核酸检测阳性标本为520份,阳性率为58.96%;EV71、Cox A16及EV未分型核酸阳性标本分别为165份(18.71%)、211份(23.92%)和144份(16.33%)。数据经统计学分析,发现3年手足口病的阳性率呈增长趋势,主要病原以EV71和Cox A16为主,2013年出现了EV未分型(CA6型)增多的情况。结论 2012年-2014年手足口病的流行呈上升趋势,应加强手足口病病原谱的监测工作,为手足口病的防控提供依据。
Objective To make a etiological surveillance and detection on hand-foot-mouth disease（ HFMD）,so as to understand the etiological characteristics of it in Xicheng district of Beijing. Methods RT-PCR was used to identify the nucleic acid extracted from the swab samples. Results The detection results showed that 520 cases were with enterovirus RNA-positive of the total 882 throat swab specimens collected during 2012-2014,and the positive rate was 58. 96%; the RNA-positive of EV71,Cox A16 and unidentified enterovirus were 165（ 18. 71%）,211（ 23. 92%） and 144（ 16. 33%）,respectively. The data were analyzed by statistics,we found that the positive rates in the 3 years was with a growth trend,and the pathogen of HFMD were mainly EV71 and Cox A16. EV unidentified（ CA6） increased in 2013. Conclusion The epidemic trends of HFMD were rising during 2012-2014,we should strengthen the monitoring of HFMD pathogen spectrum,so as to provide basis for the prevention and control of HFMD.
出处
《中国卫生检验杂志》
CAS
2015年第23期4120-4122,共3页
Chinese Journal of Health Laboratory Technology
