摘要
DNA测序技术是人类探索生命秘密的重要研究手段。自第一代的Sanger测序技术诞生以来,DNA测序技术经历了三代变革,产生了第二代到第四代测序技术,统称为新一代测序技术。目前,新一代测序技术的数据产出能力呈指数增长,而且这一技术本身也从依赖DNA聚合酶的生化反应转变为面向物理学中纳米技术的新领域。新一代测序对生命科学领域具有里程碑意义,引领了科学研究模式革新和研究思维的转变。科研人员可利用新一代测序技术对基因组、转录组和表观组等诸多领域展深入的研究。分析了新一代测序技术的特点,并对其未来的发展方向以及应用进行了展望。
DNA sequencing technology is an important research method in life science. Since the birth of the Sanger sequencing technology, DNA sequencing technology has experienced three generations of change, resulting the second generation to the fourth generation sequencing technology. DNA sequencing not only has been improving its productivity in an exponential growth rate but also been evolving into a new technological territories toward physical disciplines of nanotechnology. Next generation sequencing that has landmark significance of life sciences, can improve researches in the genome, transcriptome and epigenome. This review analyzes technical characteristics of the next generation sequencers and provide prospective insights into their future development and applications.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第11期1-8,共8页
Biotechnology Bulletin
基金
国家自然科学基金项目(31501588)
中国博士后科学基金项目(2014T70621)
山东省自然科学基金项目(ZR2013CQ018)
关键词
新一代DNA测序
边合成边测序
单分子测序
纳米孔
应用
next generation sequencing
sequence by synthesis
single molecule sequencing
nanopore
applications
