摘要
依据NCBI数据库OsPM1的序列信息,采用PCR技术扩增获取OsPM1的2 100bp的启动子序列。利用PLACE预测启动子的顺式作用元件分析表明,启动子内含有大量与胁迫相关的顺式作用元件,主要有ABA响应相关元件、脱水响应元件、低温响应元件、热激响应元件和转录因子结合元件。构建OsPM1的启动子和GUS基因融合表达载体,转入拟南芥。组织化学染色分析结果显示,非生物胁迫处理前,幼苗中GUS基因表达水平很低;干旱、低温、高盐等胁迫处理后,GUS基因表达量显著升高。研究表明,OsPM1的启动子能够显著提高在干旱、高盐和低温处理后下游基因的表达水平。
A 2 100 bp sequence of OsPM1 promoter was obtained by searching in NCBI database. The pro- moter was cloned by PCR using DNA from rice as template. Cis-acting elements in this promoter were pre- dicted by PLACE. The results showed that the promoter contains a large number of cis-acting elements as- sociated with stress,such as ABRE,DRE,LTRE, HSE and binding motif of stress-associated transcription factor. The promoter of OsPM1 was cloned into expression vector pCambia1300-221-GUS to promote the expression of GUS gene and transgenic Arabidopsis was generated. GUS activity was detected in transgenic plants after drought, cold and high salinity treatments. The expression level of reporter gene was very low without treatment. After abiotic stress treatments, such as drought,low temperature and high salt, the ex- pression of GUS increased significantly. The promoter can elicited the expression of genes downstream after abiotic stress treatments.
出处
《西北植物学报》
CAS
CSCD
北大核心
2015年第11期2179-2184,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
兵团博士资金专项(2013BB003)
石河子大学高层次人才启动项目(RCZX201218)
石河子大学育种专项(gxjs2014-yz02)
