摘要
目的研究2型糖尿病大鼠局部性脑缺血再灌注损伤时,小檗碱(BE)对AQP4表达及脑组织的保护作用。方法雄性SD大鼠随机分为假手术组、2型糖尿病大鼠脑中动脉阻塞再灌注组、假性治疗组,BE治疗组。制备脑组织切片作Nissl染色,观察各组大鼠神经行为学缺陷,采用干湿重法测定缺血侧脑半球的水肿度;蛋白印迹检测BE对缺血周边区脑组织中AQP4表达的影响。结果 BE治疗组神经功能较模型组有明显改善,神经学评分明显降低(P<0.05),皮质Nissl染色淡染区域减小(P<0.05),缺血侧脑半球干湿重比值明显减小(P<0.05),蛋白印迹检测结果显示,BE治疗组较模型组AQP4表达减少(P<0.05)。结论 BE可能通过降低AQP4表达水平降低脑水肿,从而对2型糖尿病大鼠局灶性脑缺血再灌注损伤有保护作用。
Objective To investigate the effect of berberine on the expression of AQP4 and neuronal injury after focal cerebral ischemia in diabetic rots. Methods Male SD rats were randomly divided into four groups ： control （sham surgery）, middle cerebral artery occlusion and reperfusion （MCAO/R）, MCAO/R treated with vehicle （DMSO）, MCAO/R treated with berberine. The transient focal ischemia/reperfusion was induced by in- troducing silicone-coated monofilament nylon suture from the right external carotid artery into the origin of the middle cerebral artery, which was re- moved after 60 min. In group treated with berberine, the rots were injected with berberine before and after suffered from cerebral ischemia. Similarly, in group vehicle, the animals received DMSO vehicle at the same time. The score of neurological behavior was evaluated 24 h after reperfusion. Mean- while, the rats were sacrificed for Nissl staining. To estimate cerebral edema, the wet-dry ratio was measured. The expression of AQP4 in the border of the infarct region in different groups was observed by Western blot. Results Compared with the model group, berberine improved neurological deficits（P 〈 0.05）. Berberine treatment inhibited the neuronal deformation shown by Nissl staining（P 〈 0.05）. Berberine significantly decreased the wet-dry ratio and reduced the expression of AQP4 （P 〈 0.05 ）. Conclusion These results suggested that berberine could induce neuroprotection against ischemic injury by inhibiting the expression of AQP4 in diabetic rats.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2015年第12期1102-1105,共4页
Journal of China Medical University
基金
辽宁省大学生创新创业训练计划项目
辽宁医学院校长基金-奥鸿博泽大学生科技创新基金项目
关键词
小檗碱
脑缺血
2型糖尿病
AQP4
berberine
cerebral ischemia
type 2 diabetes mellitus
AQP4
