摘要
链霉菌属是多数抗生素和化感活性物质的最重要的产生菌,而化感活性物质提取方法的研究是研究化感作用的重要步骤。以一株活性链霉菌6803(Streptomyces srtain 6803)为研究对象,以绿色化学思想为指导,设计链霉菌活性物质提取及分离为开放实验学生的研究性实验,引导学生通过查阅相关研究文献,自行设计实验方案,优选提取及纯化方法,使溶剂消耗及环境污染显著降低;高效率地分离、纯化出目标产物,并用核磁共振谱图鉴定活性物质的结构。该研究性实验设计有助于增强学生的环保意识,提高学生的实验技能及科研创新能力,从而达到提高教育和教学质量的目的。
Streptomyces is the most important genus in actinomycetes since they produce many antibiotics and biologically active compounds.The extraction of allelochemicals is an important step to study allelopathy. Extraction of allelochemicals of Streptomyces strain 6803 as the researching experimental subject is designed. The students design different experimental schemes after reading a lot of papers and carries out the experiment by themselves,the solvent consumption and environmental pollution are remarkably reduced by optimizing extraction.Optimal selection of the methods for extraction and separation can lead to efficient purification of the target compounds. The structures are elucidated by analysis of the nuclear magnetic resonance spectroscopy under the guidance.The researching chemical open experiments help the students promote their awareness of environmental protection,the operating skills and innovative ability,which can improve the quality of education and teaching.
出处
《实验技术与管理》
CAS
北大核心
2015年第11期11-13,17,共4页
Experimental Technology and Management
基金
2013年广东省高等学校教学质量与教学改革工程项目(粤教高函[2013]113-94)
华南农业大学2013年度教育教学改革与研究重点项目(JG13012)
2014广东省高等教育学会实验室管理专业委员会基金项目(GDJ2014069)
2014年广东省本科高校教学质量与教学改革工程"精品教材"立项建设项目(粤教高函[2014]97-43)
2014广东省高等学校教学质量与教学改革工程项目(GDJG20142098)
关键词
研究性实验
大孔吸附树脂
化学开放实验
绿色化学
researching experiment
macroporous adsorption resin
open chemical experiments
green chemistry
