摘要
目的用RP-HPLC-ELSD同时测定绞股蓝中人参皂苷Rb1、绞股蓝皂苷A、绞股蓝皂苷XLIX、七叶胆苷XVII的含量。方法采用Phenomenex C18（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈（A）-0.5%醋酸（B）,梯度洗脱（0～5 min,10%A;5～27 min,10%～40%A,27.1 min,10%A;27.1～30 min,10%A）,柱温30℃,流速1.0 m L/min,载气为N2,体积流量2.0 L/min,雾化温度42℃,漂移管温度65℃,增益5。结果人参皂苷Rb1、绞股蓝皂苷A、绞股蓝皂苷XLIX、七叶胆苷XVII在152.4～3048 ng（r=0.9996）、65.2～1304 ng（r=0.9998）、87.2～11744 ng（r=0.9998）、11.3～226 ng（r=0.9995）范围内的线性关系良好。平均回收率分别为100.05%、100.36%、100.82%、100.61%（n=9）,RSD分别为1.77%、2.17%、2.31%、2.65%。9批绞股蓝样品含量范围：绞股蓝皂苷A 6.92～22.63 mg/g,人参皂苷Rb1 0～11.23 mg/g,绞股蓝皂苷XLIX 0～13.24 mg/g,七叶胆苷XVII 0～4.16 mg/g。结论该方法测定绞股蓝中皂苷类成分含量灵敏、准确,分离效果较好,无干扰,可为完善绞股蓝中皂苷类成分的质量标准评价提供依据。
Objective AIM To establish the method for Simultaneous determination of Gypenoside A, Ginsenoside Rb1,Gypenoside XLIX,Gypenoside XVII in Gynostemma pentaphyllum by RP-HPLC- ELSD. Methods An Phenomenex C18column（4.6 mm×250 mm, 5 μm） was used with the mixture of acetonitrile- 0.5% acetic acid as the mobile phase in gradient elution（0～5 min,10% A;5～27 min,10%～40% A;27.1 min,10% A;27.1～30 min,10% A）. The flow rate was 1.0 m L/min.The column temperature was 30℃.Carrier gas was nitrogen with the volume flow rate for 2.0 min/L, atomization temperature was 42 ℃, drift tube temperature was 65 ℃, gain was 5.Results The calibration curves of Gypenoside A, Ginsenoside Rb1,Gypenoside XLIX, Gypenoside XVII wre in good linearity over the ranges of 152.4～3048 ng（r=0.9996）, 65.2～1304 ng（r=0.9998）,87.2～11744 ng（r=0.9998）,11.3～226 ng（r=0.9995）, respectively. the average recoveries were 98.65%（RSD of 1.95%）,99.33%（RSD of 1.72%）,99.67%（RSD of 2.28%）,100.06%（RSD of 1.98%）, respectively. content range of 9 group of gynostemma sample were that Gypenoside A for 6.92～22.63 mg/g,Ginsenoside Rb1 for 0～11.23 mg/g,Gypenoside XLIX for0～13.24 mg/g,Gypenoside XVII for 0～4.16 mg/g. Conclusion The method to determine Saponin compounds in Gynostemma pentaphyllum is sensitive, accurate, separation effect is good, It has no interference, it can provide a basis as evaluation standard for quality of Gynostemma pentaphyllum.
出处
《中国卫生产业》
2015年第15期5-8,12,共5页
China Health Industry
