摘要
利用重组乳酸克鲁维酵母(Kluyveromyces lactis)GG799表达磷脂酶A2,对其产酶发酵条件进行研究。采用单因素试验和正交试验对培养基及培养条件进行优化,确定了重组菌产酶的最佳发酵条件。结果表明:最优培养基组成为葡萄糖30 g/l、酵母粉20 g/l、蛋白胨30 g/l、Kh2PO4 3 g/l;最优培养条件为:发酵温度30℃、接种量2%(V/V)、初始pH 7.0、装液量90 ml/250 ml三角瓶、摇床转速220 r/min,在此条件下发酵培养,酶活力由(1.87±0.12)U提高到(5.35±0.27)U。
Fermentation conditions for the production of phospholipase A2（PLA2） by recombinant Kluyveromyces lactis GG799 were optimized using single factor and orthogonal design experiments. The optimal fermentation medium was composed of 30 g/l glucose, 20 g/l yeast extract, 30 g/l peptone and 3 g/l k H2PO4. The best fermentation conditions were determined as 30 ℃,2%, 7.0, 90 ml and 220 r/min for temperature, inoculum amount, initial p H, medium volume in a 250 ml flask and shaking speed, respectively, resulting in an increase of PlA2 activity from（1.87±0.12） U to（5.35±0.27） U.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第21期111-116,共6页
Food Science
基金
国家高技术研究发展计划(863计划)项目(2011AA100905)
江苏省"六大人才高峰"计划项目(2012-NY-002)
关键词
乳酸克鲁维酵母
磷脂酶A2
发酵
Kluyveromyces lactis
phospholipase A2
fermentation
